Biological Molecules

Biological Molecules - wavelength I would use would be from...

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Lab 3 Biological Molecules 1. The reagent that we used to measure sugar concentration in this lab was called 3,5- dinitrosalicyclic acid (DNS). The functional group(s) that the reagent reacted with aldehydes and ketones. 2. The reagent we use to measure protein concentration in this lab is the biuret method. Peptide bonds are the amino acid chain that it bond. 3. The protein concentration for a solution with an absorbance of 0.77 is 9.3. Cu = Cs x Au / As. Cu = 2.3 x .77 / .19 = 9.321 mg/ml Concentration in Unknown Concentration in Standard --------------------------------- = -------------------------------- Absorbance for Unknown Absorbance for Standard C 2.3 ----- = ------ C = 9.321 mg/ml 1.77 0.19 4. Yes, the patient’s urine that contained hemoglobin was patient 6. If time had permitted, I would quantify the amount of hemoglobin in this individual’s urine by using spec 20 and measure its absorbance because the concentration is proportional to the absorbance. Blank would be the distilled water. The standard would have only the hemoglobin. The
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Unformatted text preview: wavelength I would use would be from 400-500 nm. 5. The portion that is the most difficult to understand was nature of the reaction between glucose and DNS on the assay for glycosuria. What was the nature of the reaction between glucose and DNS? The nature between the glucose and DNS is that the glucose got darker and DNS stayed in the same color. 6. The proper citation for the textbook when I cite it within the text of my report is by the following order: author(s), year of publication, title, and publisher. For the textbook, I would cite it (Solomon, 2011). 7. The sections of a lab report that the implications of the diagnosis of each patient would be found in the discussion section. 8. The following statement “The presence of hemoglobin was determined by examining the specimens in front of a blank sheet of white paper” can be found in the materials and methods section....
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Biological Molecules - wavelength I would use would be from...

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