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Unformatted text preview: 4. What is a primer, and how is a primer used in PCR? Primers are required for DNA synthesis and they determine the beginning and end of a specific segment DNA to be amplified. 8. What are the three steps in a PCR cycle, and what do each do? Step 1: Denaturation, DNA strand melts into one. Step 2: Annealing, Primers bind to a single strand. Step 3: Extension, Stands elongate...
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This note was uploaded on 12/12/2011 for the course BIO 111 taught by Professor Goschke during the Spring '07 term at Cal Poly.
- Spring '07