09-final-key - Bacterial Genetics GMS6038 Final Exam Fall...

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Bacterial Genetics GMS6038 Final Exam Fall 2009 Make up a code name for yourself and put it here. ___________________________ Write down the code with your name on a sheet for the class so I can grade the exam in a blinded manner. You have 2 hours to complete the exam. Please note - this is a closed book, closed note exam. All backpacks and notebooks must be against the wall, not at your table. You may use the rest room one person at a time. Any cheating will result in a 0 for the exam and failure of the course.
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Shown on the next page is pGULIG-11. Note the following genetic elements: At the top of the map is the origin of replication of the F plasmid. Since I used both oriS and oriV in class, I have provided both names here, but they are considered to be synonymous. Moving clockwise: From 1:00-2:00 is the lacI gene expressed by its own promoter. From 2:00-4:00 the rom/rop (synonymous names) gene is expressed from the wild-type pBAD promoter which is in its natural configuration relative to the araC gene, also expressed by its own natural promoter. At 5:00 is the oriT locus of the F plasmid. At the bottom is the trp ( trpL , not trpR ) promoter driving the expression of the cat gene. At 7:00-8:00 is the repE gene expressed from the glgA gene promoter, including the glgA ribosome binding site (RBS). We did not mention the glgA gene by name in class. However, all you need to know about glgA is that it is a typical CsrA-regulated gene, meaning that it follows the most common relationship between the CsrA protein and a CsrA-regulated gene. At 9:00 is the csrA gene expressed from phage λ pL promoter. Finally from 10:00-11:00 is the csrB gene driven by the pTrc promoter. There are no other promoters on this plasmid other than those indicated on the map. Assume that all genes have appropriate translation initiation and termination sequences. The short bars crossing the circle represent typical factor-independent terminators that will prevent transcriptional read through between these different elements. Every gene that is present on pGulig-11 has been deleted from the E. coli chromosome in the strain that houses pGulig-11. The host E. coli strain also lacks the F plasmid and λ phage .
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NOTE: These questions involve 2 or 3 sentence answers - not paragraphs. The number of points is the maximum number of facts being looked for, and in some cases one fact is worth 2 points. Keep to what is being asked. Adding extra material hoping to include the right answer somewhere in the middle could count against you if your answer reveals a lack of understanding or includes incorrect information, even if unrelated to the original question. Write your answers legibly on lined paper with your code on each page. Questions 1 through 6 are based on pGulig-11.
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This note was uploaded on 12/13/2011 for the course GMS 6038 taught by Professor Gulig during the Fall '11 term at University of Florida.

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09-final-key - Bacterial Genetics GMS6038 Final Exam Fall...

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