dna-repl - Notes on Bacterial DNA Replication Here is what...

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Notes on Bacterial DNA Replication Here is what I think you should be familiar with from your reading of Chapter 1 of the text. I am assuming that you will be “re-familiarized” with the basic biochemistry of DNA replication. We’ll concentrate of things that are unique and important to bacteria, especially as they will relate to use of bacteria. 1. Table 1.1 - You should know the names and functions of the proteins/complexes, but NOT the individual components of Pol III. You should understand how these proteins work together to replicate DNA. 2. Understand the differences between DNA Pol I and Pol III, including their structures, specific functions in replication, and differences in enzyme activities. Know what the Klenow fragment of DNA Pol I is. 3. Be prepared to answer the following 2 questions in class (don’t email the answer to me) a. If you were going to sell a product to replicate DNA in tube, which polymerase would you use - Pol I or Pol III? Why? b.
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dna-repl - Notes on Bacterial DNA Replication Here is what...

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