dna07 - Proteins involved in E. coii DNA replication...

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Unformatted text preview: Proteins involved in E. coii DNA replication Protein Dnafi. o‘noa Initiator protein; primosorne {priming complex} formation DnaB dnofl DNA helicase DnaC drmC Delivers Email to replication complex SSE ssh Binding to single—stranded DNA Primase dnofi RNA primer synthesis DNA ligase lr'g Sealing DNA nicks [II NA gyros-e Supercoiling or gym Nick closing |3 gyrfl ATPase DNA Poi l pom Primer removal; gap filling DNA Pol III [hoioenzyrne contains 11 polypeptides} dnoE Polymerization dnoijgl Elf-to‘Sr editing rnhri Removes RNA primers hoiE Present in core {use} dnoN Sliding clamp doe}: Organizes complex; joins leading and lagging DNA Pol ill dnox Binds clamp loaders and 555 protein hoiA Clamp loading hoifl Clamp loading hols“: Binds SSE hoiD Binds SSE Full-length product of the riner gene. 9 Shorter product oi the rinsrX gene produced by translation-.1! I'rnniuslni'l in]; [sn- I1.- is .1. l). If — can't: -— | Figure 1.16 Structure of the mic regien of E. cuff, DnaA Shown are the pesitien of the AT-n‘ch 13nmers and I” “Ch—‘— 1 3411“ —|—— mmgnitifi'” WES— —| the position of seme ef the Dnafi. binding sequences {DnaA hexeal Also shown is an additienal Fmpertant I... p... .— -:::] {:31 [32:- -=:::] [32.» 12-prT—n‘chregien. [:bDnaAbex S’TTATCCACA 3' FAT-richu—me 5’ _TNTTNTTTT 3' Figure “LII Initiation mi repliicatjan atthe t'. Lfli'iflrigin fang region. Aha-U1 a dum1 Unmet.an Itrnteins bind to LI-n: origin, wrapping IhE! DNA around themselves and npenlng the helix. Dnac helps the Dl'ldfl- hi4 icasP m hind. Tm: Dunc. primes»;- synthesize; RNA primers, initiating replication HEB, angle-strand binding protein. Figure 1.9 Distuntinuw; tynthais of one I}? the two strands of flNA during chmmusumr replm Minn. Iii] DNA Pol ||-" replicates one strand, and the prim-ase synthesizes RNA en the ntImr swam] in the appetite direction. [2) PetE “I extend: me RNA primer to SynthEEEE an Dkazaltl [raglttrl‘lt (3] The primaw 5yn1|1c5izies another RNA primer. [4} Pei III extends. the pnrner untll It reachet the fire-anus. prim-9r. [5} F‘ul | rcmuw: the lirat RNA primer and replace: it with DNA. (6] DNAiigasr— seals the aid: 15;: Murika writinuuus Dita-9t strand, and the process» continues. The strand that is synthfiired minimum-ii}- it II‘II.“ leadlng strnr-d; the strand Hut is synthesized 1:1is-zeintinu-z'iusé‘g.r is the lagging strand. fl DWI FE! Ill 1' THE: 3 5- Primes.- 3. 5. g, and Ill 3' t- 5' 3 For I" 5' '— — Leading :lrdrIIJ FIJI I" H;— Lagging Errand H 3, 5. 3;. m "I B 5:- 3. a Pni ||| Frirnm ngasn Flgure 1.18 Termination of chromosome replication in E. coii. (A) The replication forks that start at oriC can traverse tent and terB in only one direction, opposite to that indicated by the purple arrows. (B) When they meet, between or at one of the two clusters, chromo- some replication terminates. fL is the fork that initiated to the left and moved in the counter- clock-wise direction. fR is the fork that initiated to the right and moved in the clockwise direction. oriC ,ATTACAr .ATTRCAF DnaP- helicase FLTTI'uCAT DnaB helium Flame 1-12 Thu 'Lmn'lbflne‘” model fm' hflw both me lending and lagging errands of the DNA helix mighT be sin'luitilneIerrrhg.r replicated at the replicarinn fork. SSE, sings-wand blI'IrJiF'Ig Immin. RNA primers are sham in purple {A} Pu! HI hole-enzyme is synthesiaing lagging-strand BIN-‘- from primer 2{1:I'rcledjand has iufl run min:- primer 1 . {B} Lagging- Strand WI "I (indifltfid by Shading II‘I purple} has been released 1mm the template at primer 1 and hop-Fed ahead. reassembling on :he DNA .aI Fulmer 3 {girdled} t4; synthesize an flkaaaki Fragment. Bum the Beading: anrl lagging-strand Pel III enzymes, extent for the unw LI'h-I ©© ~——-*©-a~© @ @ limmin ---Di2fl'rnin}"' E - ~13 {20 mini-- i i * initiation Chromosomes Initiatiod'l Chromosomes at on": are complete at oral? are complete 5 a. I {so min} :1 "' i R ---------- "D {W min} ---------- -- i _ .i‘ . i lnltlatI-ur: Chromosomes at uric are complete lnifiétien Chromosornfi at orilf, are compiete Figure 1.20 The timing of DNA replication during the cell cycle, with Me different generation times. The time behemn initiations {I} is the onlyr time that changes. See the tefl for definitions of i, C, and D. ! --IernIr'hethylater‘.I DNA 0 fl 0 1' s' ' ' 3' 5' J’ C E. T "A .a." 'r G t'. —h— —1-- Replicatim: Dem methylase 5* 3’ 5- 3- 5, 3, .'-- methylated DNA Hernime‘thgriated DNA fully methylated {:Nh - l-'Iethyl-ciil'ecteci mismatch repair a . ' 3' ' C U T "A .15, T C- 'L' IF.- IT. _;.... ' C E T "a. II“ T G I: . 5; 3, Figure 1.15 The methyhdlrected mismatch repair system and hmir replication create-5 hernimemylatcd DIN-'5. sequences. {A} The A in the sequence GATE is methylated cln hath. strands {1 ]. Alter rep-licm‘icu'rJ the it. in GATE; in. the new strand Is mt Immednmeejr mew-latest by the Dam meti'lylase {2. 1]. {B}Ti1e newt}; replicated DNA contains a CT mi5match (1]. The nerwa Syntheses-d strand is recognized because It is not methylated at the nearby C.th sequencer and theT in the mismatch is removed alcng with neighboring sequences [2]. The sequence is resynlhesizeci, repiacing the T with the curfect C. The neighbmlnvg GATE seqlneme ls than methyrated by the Dam methyiase (3}. The new synthesized strand is shown in purple. ...
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This note was uploaded on 12/13/2011 for the course GMS 6038 taught by Professor Gulig during the Fall '11 term at University of Florida.

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dna07 - Proteins involved in E. coii DNA replication...

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