expression07 - Gene expression in bacteria(Chapter 2 Note I...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Gene expression in bacteria (Chapter 2) Note: I am assuming that you remember the central dogma of molecular biology (DNA to RNA to protein), the basics of expression that is common to bacteria and eukaryotes (e.g., mRNA, rRNA, tRNA, rtc.), genetic code, and basics of protein biochemistry. I. Transcription A. RNA polymerase: α 2 ββ ω (core) + σ (holoenzyme) 1 . σ - binding of the core enzyme to the promoter (there are several σ s) 2 . ω - assembly of the polymerase 3. no primer needed, has helicase activity B. DNA template strand – transcribed C. DNA coding strand – same as mRNA D. gene structure for σ 70 -expressed genes: (Fig. 2.6) -35 xx -10 xx (transcription start site) xx ATG start codon TTGACA TATAAT A/G E. promoter strength – how efficiently RNA polymerase binds and initiates 1. affected by consensus, presence or absence of upstream sequences F. steps of transcription (Fig. 2.8) 1. core + σ = holoenzyme 2. promoter recognition and binding (closed complex)
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Page1 / 2

expression07 - Gene expression in bacteria(Chapter 2 Note I...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online