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Unformatted text preview: J. Theta vs. rolling circle replication and concatamers (O and P proteins) K. Role of cos site again for packaging L. How do cosmids work and what is their usefulness? III. Phage M13 A. Particle structure and genome B. Receptor C. Life cycle - Fig. 7.12 1. Early events - complementary DNA replication (RF) 2. role of Gene II 3. Rolling circle replication - Gene V D. Usefulness of single-stranded DNA phages such as M13 and F1 1. Which sites are cis active or trans active 2. Which genes can be/are provided by host cell for cloning and packaging IV. Transduction A. We have already discussed transduction in our general introduction to genetics. Look over this section for clarity/review. B. You should have a clear idea of how generalized vs. specialized occur and what the requirements for the phage are that can perform either or both....
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- Fall '11