plasmid-guide-08 - c What are the essential functions/steps...

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Bacterial Genetics and Physiology Plasmid biology and genetics What is expected of you for the unit on plasmids. 1. What is a plasmid? 2. How do plasmids replicate? (understand the 2 basic models of θ [RNA vs. protein regulators]) 3. What is host range and how is it determined? 4. What is partitioning and how is it carried out? 5. What is incompatibility and how is it determined? 6. Conjugation - a. What is conjugation? b. What are the possibilities for ability to be conjugated?
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Unformatted text preview: c. What are the essential functions/steps? d. Understand the three states of the F plasmid (F+, Hfr, F’)? e. Usefulness of conjugation in the lab. 7. Transformation a. Definition b. Natural vs. artificial transformation c. Competence - definition, functions d. Transfection - definition e. Electroporation f. Usefulness and limitations of transformation in the lab. 8. Principles of useful plasmid vectors, e.g., pBluescript, pUC, pGEX, pET, pCVD442...
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