plasmid-guide-08 - c. What are the essential...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Bacterial Genetics and Physiology Plasmid biology and genetics What is expected of you for the unit on plasmids. 1. What is a plasmid? 2. How do plasmids replicate? (understand the 2 basic models of θ [RNA vs. protein regulators]) 3. What is host range and how is it determined? 4. What is partitioning and how is it carried out? 5. What is incompatibility and how is it determined? 6. Conjugation - a. What is conjugation? b. What are the possibilities for ability to be conjugated?
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: c. What are the essential functions/steps? d. Understand the three states of the F plasmid (F+, Hfr, F)? e. Usefulness of conjugation in the lab. 7. Transformation a. Definition b. Natural vs. artificial transformation c. Competence - definition, functions d. Transfection - definition e. Electroporation f. Usefulness and limitations of transformation in the lab. 8. Principles of useful plasmid vectors, e.g., pBluescript, pUC, pGEX, pET, pCVD442...
View Full Document

Ask a homework question - tutors are online