regulation2 - H | E o «E g as*3" 3.3.6 g E s E EU...

Info icon This preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: H] | E o «E g as *3" 3 .3 .6 g E s E EU} Flmre 13.1 Growth of E. coiiin a mixture of glucose and galactose. The concentration of the sugars in the medium Is shown as the dashed lines. The optical density, a measure of cell density, is shown in black. Dnly after the cells deplete all the glucose do they begin to use the galactose, and then only aftea' a short lag while they induce the go! operon {plateau In optical density}. They then grow more slowly on the galactose. See the tout for details. Inactive adenylate cyclase 'I 3.1 Exogenous glucose inhihib both CAMP thesis and the uptake of other sugars, such as lactose. In the presence at glucose, the ratio of tin“ to IIAE'=~P high, as. glucose is phosphorylated on transport by the ose transponer, It“. Unphosphorylated Item Inhibits lactose permease, resulting in IrinclIucer exclusion." (B) in absence of glucose. the tum—P concentration is high, and activates adenylate cyclase. Also. lactose transport is itte-d. This is an ovenlmplified model of the way in ' n this complex regulation occurs in different bacteria. For complete accounts, see Deutscher et al., cited in Box .1, and Pompeo et EL, Suggested Reading. A —?U -55 "35 —'ID +1 l—l oronorfiocrcnc RNA Pol interaction region _=/m= CACTCAECGAGTG LimRNA stinding site Class | promoter Class II promoter Figure 13.! Model for CAP {CRP} activation at class I and class II CAP—dependent promoters. {A} Sequence of the CAP binding site upstream of the class I inc promoter. (B) The binding and location interactions of CAP with the C-ten'ninal and N-terrninal domains of the o: subunit with class I and class II promoteis, respectively. our site I Operator % 400 4'“ —M 41} —W —50 44} “3D —20 -Il'.'l +1 +1fl +20 +30 +40 45 raglan -1D fusion D I I a” m: _If.lr"l'lllll Flynn 135 Eegubtiunaffiuhrtunapermbyhufiglmmmeirflucwhm. {Aflimuperanhannrflyhtheabmnofgm andmepruemenfhdmwhmr: converted intuit): Inducer, ilullctuse. {B andQMop-emn lscrflln the Framed glmmflmmmtlamu present, Math: CAP-cMPcompflex i5 nutbuundtc meCAPsite.{D}Thewonkahunfifllactoselsnutmsent.mnffglucusekatmnut pmenhbecausemerepmisbumdtumeuperthemlativepmitiumufthew- binding site, wanton and mmer arc m. The entire mam region com: about mu hp nf DNA. F: F] If]; F- 11 mini-i nun-IRMA T 't ft; HI“ I333 Role Inf final in the induction ufthe heat shock games after a!!! are flFDl-Ed to an abrupt inCI'EaiE in immature or other type at stress. The a" with Bruit buund is susceptible to it pmtaase and it i:|I.rit:iI:I3.r dzgmded. In iddition. It“ with DMK baund is less active- for the hitiflim uf transaiptiun {rum flu.- hent :ih'D-Eit pmhuten. After an abrupt increase in temperature, many other protein: an: demand, and Bruit. with the I'IEip D‘i’ GrpE md Unit] in: thapter 2]. hind: ta these to hat-p them retuiti plop-adv. This attiun trees a”, stabilizing it and making it more active for trarucflptiun initiation. WI'iEI'i the cell acfium to the higher lumpy-attire and Una: mumulatcs to the point when: some is agar'n available it: bind to a", the activity at a" in the {all again drups and the transcription of H'E hunt shock gm mum: to basal ieveis. ...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern