secretion08 - Protein localization (Chapters 2 and 14). I....

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Protein localization (Chapters 2 and 14). I. cytoplasm – not a problem II. cell (inner) membrane III. exported beyond cell (inner) membrane IV. secreted outside of cell (note different use than previous years) V. translocase systems for cell membrane (Sec and Tat) VI . Sec system – General Export Pathway (GEP) A. SecY main component (SecE and SecG secondary) B. leader peptides at N terminus C. ATP driven D. posttranslational export (periplasmic, outer membrane, secreted) 1. shorter leader 2. SecB binds (prevents folding = chaperone) 3. delivers to SecA and SecYEG system 4. leader peptide cleaved by Lep releasing protein E. cotranslational (cell/inner membrane) 1. longer leader (first transmembrane domain) 2. Signal Recognition Particle (SRP) – ribonucleoprotein complex - conserved a. binds to leader peptide b. pauses translation 3. docking protein FtsY at SecYEG 4. SecA can be involved 5. energy from translation 6. YidC – helps protein thread into membrane VII . disulfide bonds a. not formed in cytosol b. directed in periplasm by DsbA (disulfide oxidoreducatase)
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This note was uploaded on 12/13/2011 for the course GMS 6038 taught by Professor Gulig during the Fall '11 term at University of Florida.

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secretion08 - Protein localization (Chapters 2 and 14). I....

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