Lecture2Viruses&Archaea

Lecture2Viruses&Archaea - Lecture 2 Marine Viruses,...

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Marine Viruses, Marine Archaea Lecture 2
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DeLong and Karl
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The tiny cube on the finger (inset) measures 1 mm on each side, a volume of one microliter. Background demonstrates the very large number of microorganisms in one microliter of seawater. The microbes have been captured on a filter, stained with a green fluorescent DNA stain, and visualized with an epifluorescence microscope. The larger green dots (numbering about 1000) are bacteria, and the much smaller dots (about 10,000) are viruses. (Courtesy Grieg Steward.)
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Viruses “Agents of microbial mortality” thus play a role in cycling of organic matter in the oceans Abundant: ~10 8 per ml in productive coastal waters; numbers correlate with system productivity, bacterial numbers and chl a Very abundant in marine nearshore surface sediments (10 8 -10 9 per cm 3 ) Enumerated by epifluorescence microscopy or flow cytometry Abundance estimates may be low if samples not flash frozen or counted immediately Contain ~200 Mt of carbon (assuming 0.2 fg of carbon in each virus), equal to carbon in 75 million blue whales Estimating “infectivity” is complicated…strain-specificity and poor representation in cultures
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Curtis Suttle, Nature 2005 Marine Phages a. Myoviruses –contractile tails, lytic, commonly isolated, broad host range
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This note was uploaded on 12/12/2011 for the course BIOL 450 taught by Professor Ms.richardson during the Fall '10 term at South Carolina.

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Lecture2Viruses&Archaea - Lecture 2 Marine Viruses,...

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