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SL+D103+lecture+8 - D103: Cell Biology Dr. Shin Lin Lecture...

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D103: Cell Biology Dr. Shin Lin
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D103 Fall 2011 Lecture 7 © Lin All rights reserved Lecture 8 The Cytoskeleton IV: Intermediate Filaments Recommended Reading: MBOC (5th edition): 1026-1030, 984-987 2
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D103 Fall 2011 Lecture 7 © Lin All rights reserved 3 Intermediate filaments A. Function and structure of IF Heterogenous group of proteins Impart mechanical strength to cell B. Sample IFs 1. Keratins and their role in tissue maintenance 2. Lamins and their role in nuclear organization
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D103 Fall 2011 Lecture 7 © Lin All rights reserved 4 NOT conserved : not found in all eukaryotes • heterogenous : tissue specific - several proteins with different amino acid composition which share overall protein organization • subunits form lateral self association NO filament polarity due to head-to-tail subunit alignment Intermediate Filaments
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D103 Fall 2011 Lecture 7 © Lin All rights reserved 5 The overall organization of intermediate filaments
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D103 Fall 2011 Lecture 7 © Lin All rights reserved 6 Model of intermediate filament construction Intermediate filament construction→ final 10nm rope-like filament
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D103 Fall 2011 Lecture 7 © Lin All rights reserved 7 This PtK2 (epithelial) cells were stained for mitochondria network with MitoTracker CMXRos ( Red ), anti-cytokeratin followed by fluorescein conjugated secondary antibody for intermediate filaments (green) , and DAPI for DNA ( blue ) Intermediate Filaments and Mitochondria Visualized by Fluorescence Microscopy
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SL+D103+lecture+8 - D103: Cell Biology Dr. Shin Lin Lecture...

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