Exam 2 answers

Exam 2 answers - • Protein Product multiple AUG start...

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Question 1: dnaA starts replication oriC - Made up of a triple repeated AT rich region AND a DnaA binding region with lots of repeats. DnaA(s) bind and cause the formation of a loop. Localized supercoiling causes the triple repeat region (DUE) to unwind. DnaC (with ATP) loads DnaB onto the unwound part DnaB is the helicase used in DNA Pol (other proteins like HU are also somehow used, unknown…)
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Question 2: understanding tRNAs
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Question 3: Okazaki Fragments in Replication Forks
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Q4: Prokaryotes mRNA
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Q4: Eukaryote RNAs
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Q5: DNA rep is semiconservative
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Q6: Codons and Anticodons Codon 5’ ACU 3’ could pair with anticodon: 3’ UGA 5’ 5’ AGU 3’ 3’ UGG 5’ 5’ GGU 3’ 3’ UGI 5’ 5’ IGU 3’ Always remember they are written 5’->3’ when not told otherwise!
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Q7: eukaryotic genes in bacteria Transcription: - promoters are all wrong. - transcription terminator? Rho or hairpin? Translation: - ribosome binding site (SD sequence)? - codon usage (reduced translation)
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Unformatted text preview: • Protein Product?- multiple AUG start positions make other things?- did introns screw it up? (they don’t work in prokaryotes)- post-transcriptional modifications? Q8: Requirements for Translation • Ribosomal assembly requires three IFs. • IF-2 needs one GTP • Always uses fMet for the start Q9: mapping codons and amino acids • Degenerate: Most amino acids can be specified by more than one codon • Non-ambiguous: A codon maps to exactly ONE amino acid • Universal* (some exceptions apply • Read in triplets, unpunctuared, nonovelapping • One start, three stops Q10: Q11: rRNA in ribosomes • Ribosomes:-30S + 50S = 70S- Lots of rRNAs mixed with proteins • 16S rRNA:- The 3' end contains the anti-Shine-Dalgarno sequence which binds upstream to the AUG start codon on the mRNA. • 23S rRNA:- acts as a ribozyme- “peptidyl transferase”...
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Exam 2 answers - • Protein Product multiple AUG start...

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