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tutorial2_ans - Bioc 300 Tutorial 2 July 14 th 2009...

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Unformatted text preview: Bioc 300 Tutorial 2 July 14 th 2009 Topics:- Enzyme thermodynamics, michaelis-menten kinetics, enzyme inhibition, chymotrypsin, hemoglobin. 1. Determination of the precise spacing of atoms within a large protein is possible only through the use of: a) a Ramachandran plot. b) molecular model building. c) x-ray diffraction. d) light microscopy. e) electron microscopy Ramachandran Plots a way to visualize dihedral angles against of amino acid residues in protein structure. shows the possible conformations of and angles for a polypeptide. X-ray Diffraction The pattern of how x- rays scatter when they hit a crystallized protein can be used to determine the location of the atoms 1. Determination of the precise spacing of atoms within a large protein is possible only through the use of: a) a Ramachandran plot. b) molecular model building. c) x-ray diffraction. d) light microscopy. e) electron microscopy Michaelis-Menten Dynamics Describes the kinetics of simple enzymes Let the forward and reverse be in stead state (ie equal) Initially, we have the following system: V o = k 2 [ES], (and Vmax = K2[E total ] Graphing Michaelis-Menten Kinetics Graphs are known as LineweaverBurk plots Really just a double reciprocal of the V o expression (and rearranged into the form y = kx + c) Effects of Inhibitors 2. In competitive inhibition, an inhibitor?...
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tutorial2_ans - Bioc 300 Tutorial 2 July 14 th 2009...

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