tutorial5 - involves-All required reagents and products...

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Biochem 300 -Tutorial 5 July 23 rd 2009 Topics: E.T.C., glycogen storage and gluconeogenesis, lipids
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1. [Problem Set] Given the following table of standard reduction potentials: Calculate the change in Gibb’s free energy that results from transferring two electrons from QH 2 to O 2 .
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2. [Problem Set] Consider the following scenario: A sample of glycogen from a patient with liver disease is incubated with orthophosphate, phosphorylase, the transferase, and the debranching enzyme (α1-6-glucosidase). The ratio of glucose 1- P to glucose formed in this mixture is 100. What is the most likely enzymatic deficiency in this patient?
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3. Which molecule can NOT be used as a precursor for gluconeogensis in the liver? a) Pyruvate b) Lactate c) Acetyl-CoA d) Oxaloacetate e) Aspartate f) Succinyl-CoA
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4. Consider the enzyme Pyruvate Carboxylase? a) What is the reaction catalyzed by this enzyme and include the following: - The structures of the major molecules
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Unformatted text preview: involves-All required reagents and products (try to draw it out) b) Where does this occur in the cell c) When is this enzyme used? d) What is the cofactor used by this 5. The three enzymes used in glyconeogensis but NOT in glycolysis are:-Fructose 1,6-bisphosphatase-Phosphoenolpyruvate Carboxkinase-Pyruvate carboxylase How are each of these 3 bypass reactions regulated? 6. Understanding Lipids: a) Draw the structure a simple triacylglycerol composed of oleate (oleic acid), palmitate (palmitic aci), and linolenate (linoleic acid) (in any order). b) Do you think this triacylglycerol would have a higher or lower melting point than one composed entirely of palmitates? 7. Energy Storage a) What are the two primary ways energy is stored in the cell? b) Which is better, ie provides more bang for your buck unit-wise. (provide at least two reasons.)...
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This note was uploaded on 12/13/2011 for the course BIOLOGY 300 taught by Professor Asdf during the Spring '08 term at The University of British Columbia.

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tutorial5 - involves-All required reagents and products...

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