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tutorial6 - Bioc 300 Tutorial 6 Biochem 300 tutorial 6 July...

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Bioc 300 – Tutorial 6 Biochem 300 – tutorial 6 – July 28 th 09 Topics: lipids, fatty acid synthesis and beta oxidation
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1. Important reactions in fatty acid synthesis. a) What enzyme catalyzes the committed step of fatty acid synthesis? b) Write out the 2-step reaction catalyzed by this enzyme, providing names of reactants, intermediates, and products
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2. Cost analysis for fatty acid synthesis. a) How many acetyl-CoA used for initial priming of the enzyme? ________ b) How many acetyl-CoA used for synthesis of each malonate? __________ c) How many malonate used (how many reaction cycles) per synthesis of one 16-C palminate? ________ d) Total acetyl-CoA used for priming and for syntheisis of malonate, a + b(c): ________ e) How many ~P bonds of ATP used for synthesis of each malonate? __________ f) Total ~P bonds of ATP used for synthesis of one 16-C palmitate, 2a(1c): ________ g) How many NADPH used per reaction cycle? __________ h) Total NADPH used per synthesis of one 16-C palmitate, 3a(1c): _________ a) Write a balanced equation for synthesis of palmitate from acetyl-CoA, listing net inputs and outputs:
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3. Which of the following is true about
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