Bio200_20Dec2006_ans_key - Name(Last name first Student...

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1 Name (Last name first): _______________________________________ Student Number:_____________________ Biology 200 Final Exam - Dec. 6, 2006 1. Write all answers on the exam in PEN. Do not use pencil or red pen. 2. Write your name and student registration number on this exam AND on the Exam booklet. 3. You will have 2.5 hours to take this exam. 4. Place your student card on the table in front of you for checking during the exam. 1 2 3 4 5 6 7 8 9 10 11 Total 8 5 9 6 12 5 8 6 10 6 25 100 1. (8 marks) A. (5 marks) Shown below is a diagram of a typical eukaryotic gene. Draw a diagram for the mature mRNA that would result from transcription of this gene and RNA processing. Indicate if there are any modifications. Indicate the 5’ and 3’ end. Answer in exam booklet. 1 mark for 3’ and 5’, 2 marks for getting introns out/exons joined, 1 mark each for cap and tail. B. (3 marks) Is this the only mature mRNA that can result from this transcript? Explain. Write answer in exam booklet. No (1 mark), after this gene is transcribed the transcript can be alternatively spliced (2 marks). For example, exon 2 could also removed during splicing. The resulting mature mRNA will contain only Exon 1 and Exon 3. 2. (5 marks) Gut epithelia cells contain the Na+/glucose symport to import glucose across the plasma membrane. Where does the energy come from to move glucose across the plasma membrane? Write answer in exam booklet. The energy comes from ATP (1 mark) which is hydrolyzed by the Na+/K+ pump (2 marks) as it pumps Na+ out of the cell and K+ into the cell. The resulting Na+ gradient represents a form of stored energy and is used to pump glucose against its concentration gradient (2 marks). 3. (9 marks) Phospholipids form sealed spherical vesicles (liposomes) in water. Assume that you have constructed liposomes that contain ATP synthase with the portion of the protein that normally faces the promoter start codon stop codon terminator INTRON 1 INTRON 2 EXON 1 EXON 2 EXON 3
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2 mitochondrial matrix oriented towards the outside of the liposomes. What would happen if (Write answer in exam booklet.) : a) your liposomes were suspended in a solution containing the same pH as the solution inside? Explain briefly. Nothing happens because no concentration gradient to do work (2 marks) b) you add ATP to the solution described in a? Explain briefly. ATP synthase can work in reverse to pump H+ into the liposome using ATP hydrolysis (3 marks) C. You make the solution outside the liposomes more basic than the solution inside and provide necessary substrates? Explain briefly. ATP could be synthesized (2 marks), powered by the H+ or pH gradient (2 marks)
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Bio200_20Dec2006_ans_key - Name(Last name first Student...

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