final 2 key[2]

final 2 key[2] - 1 Name (Last name first): _ Student...

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Name (Last name first): _______________________________________ Student Number:_____________________ Lecture Section __________ Biology 200 Final Exam-2 Dec. 16, 2008 1. Write all answers on the exam in PEN. Do not use pencil or red pen. 2. Write your name and student registration number on this exam AND on the Exam booklet. 3. You will have 2.5 hours to take this exam. 4. Place your student card on the table in front of you for checking during the exam. 1 2 3 4 5 6 7 8 9 10 Total 6 5 6 5 8 5 9 6 8 30 88 Question 1. (6 marks) For the following questions, circle the correct answer. There may be more than one correct answer for each question. a. (2 marks) Circle all of the following that are features of Watson and Crick’s model of DNA: a. the nucleic acid chains run parallel to one another (5' to 3' matches 5' to 3') b. it follows rules of base pairing: A pairs with T, G pairs with C. c. purine covalently binds to pyrimidine bases. d. backbone consists of phosphodiester bonds between deoxyribose sugars e. backbone consists of phosphodiester bonds between ribose sugars f. backbone consists of nitrogenous bases b. (2 marks) If kinetochore microtubules on one side of a metaphase chromosome are destroyed during metaphase, both chromatids would: a. stop in place b. stop in the center of the cell c. attach to another microtubule d. move to opposite spindle poles e . move to the same spindle pole c. (2 marks) What directly controls the activity state of cyclin-dependent kinase? a. ATP binding b. GTP binding c. phosphorylation and dephosphorylation d. cyclin binding e. tubulin 1
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Question 2. (5 marks) Unlike mitochondria, chloroplasts do not have a transporter that allows them to export ATP to the cytosol. How then does the rest of the plant get the ATP it needs? Chloroplasts use photosynthesis to capture light energy and produce ATP, which is then used inside the chloroplast to produce carbohydrates (sugars) for export to the cytosol (2 marks). Those carbohydrates, e.g. pyruvate, are oxidized in the mitochondria and ATP is made in the mitochondria by oxidative phosphorylation (3 marks). Question 3. (6 marks) Imagine that you have engineered genes, encoding one protein with multiple targeting sequences. State where the protein would be located after targeting if the protein was produced by a cell. Briefly, explain your reasoning. a) (2 marks) One signal for import to the nucleus and one for import to the ER. The ER (1 mark) Proteins are inserted into the ER by co-translational insertion. Nuclear proteins are imported to the nucleus only once they are completely translated and folded (1 marks). b) (2 marks) One signal for import into the ER, one for transfer to a lysosome, one for retention in the ER lumen. The ER signal is required to import the protein into the ER.
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final 2 key[2] - 1 Name (Last name first): _ Student...

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