final keyDec. 6, 2008

final keyDec. 6, 2008 - 1 Biology 200 Final Exam-1 Dec. 6,...

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Biology 200 Final Exam-1 Dec. 6, 2008 1 2 3 4 5 6 7 8 9 10 Total 4 6 5 6 6 6 10 10 5 30 88 Question 1. (4 marks) a. What type of microscopy was used? (2 marks) __ Transmission Electron Microscope (TEM) __ b. Identify the cytoskeletal element indicated by the arrow. (2 marks) _________ Microtubules _________ Question 2. (6 marks) For each of the following, circle the correct answer.There may be more than one correct answer for each question: a. (worth 2 marks – all or none) Check all of the following that are features of Watson and Crick’s model of DNA: a. backbone consists of noncovalent bonds between deoxyribose sugars b. the nucleic acid chains run antiparallel to one another (5' to 3' vs. 3' to 5') c. it follows rules of base pairing: A pairs with T, G pairs with C. d. purines always hydrogen bond with pyrimidines. e. backbone consists of phosphodiester bonds between deoxyribose sugars f. backbone consists of phosphodiester bonds between ribose sugars g. backbone consists of nitrogenous bases b. (2 marks) What keeps the Golgi apparatus in the middle of the cell, and away from the PM? a. Intermediate filaments b. Kinesin c. Dynein d. Myosin e. Actin c. (2 marks) What directly controls the assembly/disassembly state of lamins? a. ATP b. GTP c. Phosphorylation d. Proteolysis e. NLS 1
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Question 3. (5 marks) In the space provided, explain in one or 2 sentences why plants need to have mitochondria in every cell, even when the sun is shining. Mitochondria use breakdown products from carbohydrates produced by chloroplasts to form ATP (oxidative phosphorylation) for the cell's use (2). Even in photosynthetic cells, where chloroplasts capture light energy and make ATP, that ATP is used in the dark reactions/Calvin cycle to produce sugars (2), which are then exported to the cytosol, and later on to the mitochondria (1). Question 4. (6 marks) Circle, on the diagram below, two places where receptor recycling occurs. Give an example of the receptor and its cargo for each of them. 2 marks: for circles on diagram. 1 mark each for Receptor and Cargo/ligand = 4 marks total Question 5. (8 marks) Based on the hydropathy plot of the primary sequence of a plasma membrane protein, predict how the protein will be arranged in the membrane. Use a well-labeled diagram including PM, cytosol, extracellular space, N and C termini. 2 1. Receptor_ LDL, insulin (Plasma membrane receptors) Cargo/ligand__ receptor mediated endocytosis, (e.g. LDL, insulin) ____ 2. Receptor__ Mannose-6- phosphate __ Cargo/ligand__ Lysosomal enzymes
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Question 6. (6 marks) This is a liposome containing an ATP synthase (arrow) and a light-driven proton pump (arrowhead). Given all of the reagents and light, this liposome system will produce ATP. a. There wouldn’t be a proton gradient (2 marks), and therefore no ATP
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final keyDec. 6, 2008 - 1 Biology 200 Final Exam-1 Dec. 6,...

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