MBB222-lecture9

MBB222-lecture9 - Protein Function General Strategies for Enzyme Action only a few residues in the active site participate in catalysis these are

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Protein Function General Strategies for Enzyme Action
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• only a few residues in the active site participate in catalysis - these are typically residues that are distant in the primary sequence but come together in the 3D folded structure of the protein.
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• the active site residues typically form multiple, weak, yp y p , , noncovalent interactions with the substrate • these combined interactions are highly specific and short- range, requiring molecular complementarity between the substrate and the active site e full complement of these interactions is formed only the full complement of these interactions is formed only during the transition state
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• the bonds between the enzyme and the transition ate substrate adds state substrate adds stability and thus lowers the activation energy • ‘ES’ and ‘EP’ represent lower energy termediates in the intermediates in the reaction pathway
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Enzyme Kinetics: • study of the rates of chemical reactions • if we graph reaction velocity vs. bstrate concentration for varying substrate concentration for varying amounts of substrate and a fixed amount of enzyme – results in hyperbolic curve – initial slopes are very steep, but en begin to plateau then begin to plateau, approaching a maximal value • reaction velocity refers to the change in substrate concentration with respect to time
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• Leonor Michaelis and Maud Menten proposed a simple model to account for these characteristics ok into account the enzyme bstrate complex (ES) – took into account the enzyme-substrate complex (ES) as a necessary intermediate in catalysis – E + S ES E + P – as more substrate is added, the enzyme becomes saturated with substrate (i.e., is working at full
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This note was uploaded on 12/13/2011 for the course MBB 201 taught by Professor -- during the Spring '11 term at Simon Fraser.

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MBB222-lecture9 - Protein Function General Strategies for Enzyme Action only a few residues in the active site participate in catalysis these are

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