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CHE 339/BME 339/BIO 335
Exam I
Thursday, October 2, 2008
Your name: ________________________________
EID: _____________
Part 1: Short Questions:
ANSWER IN THE SPACE PROVIDED
1.
Cell growth (12 points total)
A. Name 4 ways for measuring cell growth
(4 points).
B. Write the relationship that describes how the growth rate (or the cell death rate constant)
varies as a function of T?
(2 points)
C. What is the Crabtree effect?
(3
points)
D. Explain briefly the molecular basis for the Crabtree effect, i.e. why it happens
(3 points)
2.
Monod Kinetics (8 points total)
Your boss asked you to determine the
μ
max
and Ks values for the organism
Longhornious
pestis
which was recently discovered at the Royal Memorial stadium.
You have in your
disposal shake flasks, growth media (you wish to grow the bacteria with glucose as the
limiting nutrient) and standard microbiological equipment (but no continuous stirred tank
fermenter).
Describe what experiments you would need to do, the data you will have to
obtain and how you will plot the data to calculate the parameters you need
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Chemostats (10 points total)
A.
In an ideal chemostat we assume that the Yield coefficient is constant and independent of
the dilution rate but in reality we have to take into account the maintenance energy
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This note was uploaded on 12/13/2011 for the course BME 339 taught by Professor Georgiou during the Spring '11 term at University of Texas at Austin.
 Spring '11
 Georgiou

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