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Exam I 2008 - CHE 339/BME 339/BIO 335 Exam I Thursday...

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CHE 339/BME 339/BIO 335 Exam I Thursday, October 2, 2008 Your name: ________________________________ EID: _____________ Part 1: Short Questions: ANSWER IN THE SPACE PROVIDED 1. Cell growth (12 points total) A. Name 4 ways for measuring cell growth (4 points). B. Write the relationship that describes how the growth rate (or the cell death rate constant) varies as a function of T? (2 points) C. What is the Crabtree effect? (3 points) D. Explain briefly the molecular basis for the Crabtree effect, i.e. why it happens (3 points) 2. Monod Kinetics (8 points total) Your boss asked you to determine the μ max and Ks values for the organism Longhornious pestis which was recently discovered at the Royal Memorial stadium. You have in your disposal shake flasks, growth media (you wish to grow the bacteria with glucose as the limiting nutrient) and standard microbiological equipment (but no continuous stirred tank fermenter). Describe what experiments you would need to do, the data you will have to obtain and how you will plot the data to calculate the parameters you need
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3. Chemostats (10 points total) A. In an ideal chemostat we assume that the Yield coefficient is constant and independent of
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