Exam I 2009 - Name: _ EID:_ CHE 339/BME 339/BIO 335 Exam I...

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Name: _______________________ EID:____________________ CHE 339/BME 339/BIO 335 Exam I Thursday, October 1, 2009 ANSWERS IN PENCIL CANNOT BE SUBMITTED FOR REGRADE Part 1: Short Questions: ANSWER IN THE SPACE PROVIDED 1. Microbial growth [ 9 points total] A. Show how microbial growth varies as a function of time in a batch fermentation (e.g. in a shake flask) and name the four main phases of growth. (5 points) B. What are secondary metabolites and when do they form (2)? C. Describe (one sentence each) quorum sensing and balanced growth (2) Points: /9
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Name: _______________________ EID:____________________ 2. Explain the molecular basis of the Crabtree effect? Show a plot of μ vs. S that shows what happens when the Crabtree effect occurs ( 4 points ) 3. Chemostats [ 9 points total] A. For an ideal chemostat we assume that the Yield coefficient is constant and independent of the dilution rate, but in reality we have to account for the cellular maintenance energy requirement. Derive an expression for obtaining the maintenance energy requirement given a set of values of the observed yield coefficient, Yx/s, and the dilution rate, D (7 points) B. Show on a graph how limitation for the nitrogen source in a continuous stirred tank fermenter affects the X vs D plot relative to an ideal (glucose-limited chemostat) (2 points) Points: /13
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This note was uploaded on 12/13/2011 for the course BME 339 taught by Professor Georgiou during the Spring '11 term at University of Texas at Austin.

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Exam I 2009 - Name: _ EID:_ CHE 339/BME 339/BIO 335 Exam I...

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