Exam I - CHE 339/BME 339/BIO 335 Exam I Thursday October 7 2010 Your name ANSWERS IN PENCIL CANNOT BE SUBMITTED FOR REGRADE Part 1 Short Questions

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CHE 339/BME 339/BIO 335 Exam I Thursday, October 7, 2010 Your name ________________________________ ANSWERS IN PENCIL CANNOT BE SUBMITTED FOR REGRADE Part 1: Short Questions: ANSWER IN THE SPACE PROVIDED 1. Cell growth (20 points total) A. Name 4 ways for measuring cell growth (4 points). - Direct Counting - Dry cell weights - Turbidity measurements - Indirect measurements (metabolites, COM) B. Briefly (no more than 5 words each) describe 4 important biochemical events that occur when cells enter stationary phase (6 points). - Sigma factors (change of genetic profiles) - Nutrient accumulations - Quorum sensing - Secondary metabolites (i.e. antibiotics) C. What are secondary metabolites and why are they important? (2 points) Secondary metabolites are not related to primary metabolism nor involved in normal growth/cell viability. They are formed during stationary phase. D. Your boss asked you to determine the μ max Ks and Y x/s values for the organism Longhornious pestis which was recently discovered at the Royal Memorial stadium and was shown to grow with glucose as the C source. You have in your disposal a continuous stirred tank fermenter, growth media, a spectrophotometer and a kit that allows you to measure the conc of glucose in a solution. Describe what experiments you would need to do, the data you will have to obtain and how you will plot the data to calculate the parameters you need (8 points). Use chemostat to grow bacteria at various inlet substrate levels (Sin) and dilution rates (D) Measure Sout (S) at exponential phase After obtaining the various data points [substrate levels (S), dilution rates (D)], plot their inverse values from the Monod Kinetics model to fit the Lineweaver Burk equation 1/D = Ks/umax * 1/S + 1/umax Y-intercept gives 1/umax and Slope gives Ks/umax ---> Ks Measure cell mass out (X) and Sout (S) during exponential phase and use X=Y X/S (Sin-S) to get Y X/S
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2. Oxygen transfer [8 points total] A. How does the Oxygen Transfer Rate (OTR) depend on the interfacial area and the driving force for mass transfer (i.e. write the equation and explain the meaning of the terms
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This note was uploaded on 12/13/2011 for the course BME 339 taught by Professor Georgiou during the Spring '11 term at University of Texas at Austin.

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Exam I - CHE 339/BME 339/BIO 335 Exam I Thursday October 7 2010 Your name ANSWERS IN PENCIL CANNOT BE SUBMITTED FOR REGRADE Part 1 Short Questions

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