Exam I 2011 - CHE 339/BME 339/BIO 335 Exam I Thursday,...

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CHE 339/BME 339/BIO 335 Exam I Thursday, October 6, 2011 Your name and UT EID ________________________________ ANSWERS IN PENCIL CANNOT BE SUBMITTED FOR REGRADE Part 1: Short Questions: ANSWER IN THE SPACE PROVIDED 1. Cell growth (20 points total) Elemental Composition (C, N, P) and Cell Volume of Exponentially Growing and Nutrient-Limited Bacterioplankton Katarina Vrede, Mikal Heldal, Svein Norland, Gunnar Bratbak, Appl Environ Microbiol. 2002 June; 68(6): 2965 2971. doi: 10.1128/AEM.68.6.2965-2971.2002 A. You and your research captain sampled s eawater from Raunefjorden, 15 km south of Bergen, Norway (60°16 N, 5°14 E), in October 1997. Marine bacterioplankton was isolated and grown in batch cultures. Write the generic equation for specific microbial growth rate. (4 points) B. All four isolates were grown in a medium so that their growth finally became limited by organic carbon. Samples for X-ray microanalysis were taken during exponential growth and at stationary phase. Briefly (no more than 5 words each) describe 4 important biochemical events that occur when cells enter stationary phase. (4 points)
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C. The elemental contents of individual bacterial cells were analyzed with a Joel 100CX transmission electron microscope equipped with a Tracor Z-MAX 30 X- ray detector featuring silicon crystal and Norvar single window for light element detection. The elemental composition of the bacterioplankton was determined to be: CH 1.6 O 0.55 N 0.25 P 0.1 . The carbon source is glucose (C 6 H 12 O 6 ), the nitrogen source is ammonium hydroxide (NH 4 OH) and the phosphorous source is HPO 4 . The respiratory quotient is 1.2. Your research captain asked you to set up the equations you will need to solve for the stoichiometric coefficients. Assume all nitrogen and phosphorus is incorporated into the bacterioplankton. She did not ask you to solve them because the company has a computer algorithm that will solve the equations once they are set up. (8 points)
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D. Interestingly, you found that the growth rates changed and even the
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Exam I 2011 - CHE 339/BME 339/BIO 335 Exam I Thursday,...

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