Exam II 2009_solution-2pgs

Exam II 2009_solution-2pgs - SECOND EXAM CHE7339/BIO...

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Unformatted text preview: SECOND EXAM CHE7339/BIO 33SIBME 339 FALL 2009 Tuesday, November 10, 2009 YOUR NAME: ‘ BID: 1. The gene fiatemity is thought to be responsible for a disorder characterized by complete lapse of mental function in young adult males, particularly following moderate alcohol consumption. [15 points total] Questions A , B, and C are independent ofeach other! \U/U/ A. The flatemity gene is expected to contain at least two introns. Describe how you would make a suitable library that will allow you to isolate the protein coding sequence of the gene. (5 points) chA’ “MAM ’VW (RNA VOA/ML “WW“ I - . NA mun iv MW” ’l / mifbwsib‘m» o\ h (IMO Camp» 5'67 " Jpn/6v} vvfl’k SS MP: mm“ \1/ \w B. You Wish to clone the gene. Earlier studies at Texas A&M identified a small fragment of DNA that is knoWn to be part of the FRATERNITY gene. Briefly describe (in three steps) how you would isolate the gene from the library you made in A. (5 points) robe DNA swwnmfl WW” P g 6) Wait mum/m on duskjmoxtevoeuct cannula MM @ WWW HHW Nth Moud’wi 1?“ , @wx wimmwmw Wm «gum +04%th WWW" . COWVHM W WM. ‘ ~' \0/ k ’6 C. After you clone the gene in bacteria you are asked to perform a Noithernand a Enid/{Am points) bNPv YFObHuj {W bNh I)!” ‘ WWI] .. (23¢ anhbom”. 1. -mm PWRW’ '2. The gene for the mammalian pyruvate dehydrogenase enzyme has been cloned in E. 0011'. WW ' It was. confinned by hybridization experiments that the bacteria synthesize mRNA for the: \D mammalian 'pyruvate dehydrogenase. HoweVer, no pyruvate' dehydrogenase- Enzymatic q/o “(V activig'was found in the recombinant cells. You know that the gene construct you areusing Qfl‘ has a Shine Dalgamo sequence. [10 points total] ASuggest four possible explanations for this result (6 points) «RN/5: s—lemLoops smchfe > Sllwi’mu‘l‘a/CWOV‘ 090cm " WW3 NM?) ——.> 0% «Pct/owns - 'mmm dc? maul/“W gown—case alcha \ C For two of these possible explanations outline how you can go abdut fixing the problem. (4 points) * - . , : \eclwe \L 3 m (in one sentence each) four ways for introdflcing DNA into cells. [8 points] 1 Cad; hamshymmfiohv CM and 8m Mac/PW mmbrahz z Emmvomhon. elemental mmwwhon Ki “(i/03:23? QNWN WWt Vows L~3kV) lapping MW 3 wal 4—0 ml ton mg \Ith/Sr/S Wm “"WLP’ LL: Pav’nclc gun» shoot DNA mowwm—s WWO mu 5 thpLMl’s é’mwmj oflleMl [h WK [DNA \fiM'PLMWM WIMLMWL Mohwes A“ _ ‘ (”LOASVSKbM M’MVfl ...
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