Multiple_Choice - SECTION II Multiple choice(15...

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Unformatted text preview: SECTION II Multiple choice (15 points, 1 point per question) 1. Sometimes proteins are separated by liquid ­liquid extraction. This means that: A. First you add one organic solvent to separate the protein and then a second solvent. B. You mix the protein with water miscible organic solvents such as butanol. C. You generate an aqueous 2 ­phase solution by adding a polymer like PEG and a second component which could be either a salt or another polymer. D. You simply add starch to the protein solution and the starch separates from the proteins. 2. Which method cannot be used to break yeast. A. A blender usually containing steel beads for more impact. B. Lipase enzymes that hydrolyze the cell membrane. C. A sonicator that generates ultrasound. D. High pressure homogenizer E. None of the above; all these methods will work just fine. 3. The Crabtree effect is: A. Formation of products such as antibiotics in stationary phase. B. A way for the cell to get rid of excess NADH by producing ethanol or acetate. C. The consumption of substrate to needed to maintain the cell viable. D. Describes the effect of salts on protein solubility. 4. A higher T during sterilization is preferable because: A. Contaminants are destroyed faster. B. The activation energy for the destruction of nutrients is higher than that of cells. C. There is less destruction of nutrients. D. All of the above. 5. Which of the following methods can be used to determine the amount of a specific mRNA (e.g. interleukin receptor 2) in cells: A. Northern blotting. B. Gene chips (DNA microarrays). C. High throughput sequencing of cDNA D. All of the above (A, B and C). E. Southern blotting 6. To recover correctly folded proteins from inclusion bodies it is necessary to: A. Denature the protein using urea and then remove the denaturant by dilution to allow it to fold into its native conformation. Denature the protein and then separate the folded protein by chromatography. C. Incubate the inclusion bodies with chaperones and ATP . D. Use a detergent to dissolve the aggregate E. All of the above. B. 7. What are the essential parts of a plasmid cloning vector? A. An origin of replication, a gene that allows selection of cells transformed with the plasmid and convenient restriction sites. B. An origin of replication, a gene that allows selection of cells transformed with the plasmid and a promoter followed by a ribosome binding site. C. A means for packaging the DNA into a virus. D. An origin of replication for E.coli, a gene that allows selection of cells transformed with the plasmid and second origin of replication for a different host cell such as yeast. 8. Which part of plant biomass is most difficult to degrade biologically? A. Cellulose B. Hemicellulose C. Lignin D. Starch E. Cell wall glucans 10. “Humanization” is: A. A process for isolating human stem cells B. The engineering of proteins and, in particular, antibodies to remove sequences that can elicit an immune response when injected into a human C. The generation of hybrid cells (cell fusions) between human B cells and mouse myeloma cells D. None of the above 11. In the hybridoma technology: A. B cells are grown in bioreactors to produce antibodies B. B cells are immortalized by infecting with viruses C. B cells are immortalized i.e. are made to grow for many generations by fusion to cancer (myeloma) cells D. T cells are used to stimulate antibody production in the test tube 12. A molecular beacon is: A. An antibody that is conjugated to an enzyme and used for protein detection. B. A DNA molecule that is used for the fluorescent detection of a target DNA C. A DNA molecule that is used in the OLA assay to detect single nucleotide mutations in DNA. D. The process by which light is emitted during the incorporation of bases in high throughput DNA sequencing. E. An antibody labeled with a radionucleotide that is used to detect tumors in the body. 13. Subunit vaccines are: A. Safer than attenuated vaccines A. Consist of immunogenic components from a pathogen. C. Have a long self ­life D. Usually have to be used with an adjuvant E. All of the above. F. A and D only. 14. Very large genes (> 15 Kb) for genome engineering can be constructed by: A. Gene synthesis machines B. Cloning and ligating smaller fragments C. Overlap PCR of 0.5 to 1 kb fragments D. By C above and by taking advantage of recombination in yeast. E. All of the above. F. A, C and D 15. Yeast artificial chromosomes (YACs): A. Are used routinely for cloning in yeast B. Contain the 2 mm origin of replication and a selectable marker C. Contain a yeast kinetochore region. D. Are used for cloning very large pieces of DNA E. Are not stably maintained in yeast cells and therefore progeny cells not containing YACs arise at a high frequency. ...
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This note was uploaded on 12/13/2011 for the course BME 339 taught by Professor Georgiou during the Spring '11 term at University of Texas.

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