Problem solving

# Problem solving - Lecture 9 CHE/BME339 Take a break Review...

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Lecture 9 CHE/BME339: Take a break: Review Problem Solving What is the purpose of a phase I clinical trial? Lecture 8: safety with healthy people Name three advantages of recycle continuous stirred tank reactors? Name 4 ways for measuring cell growth: Lecture 3: turbidity, direct count, measuring output of product proportional to growth (indirect measurement of cell mass), direct measurement of cell mass Describe yield coefficient: Lecture 3 change of cell mass per change in substrate, may vary over time What is growth associated product? Lecture 3: related to primary metabolism Experimentally how do you determine the mass transfer coefficient for oxygen transfer. Homework 3 What are the disadvantages of a batch reactor? Diffficult to control, nutrient depletion, downtime, not good for research app for characterizing growth Assumptions for chemostat? Lecture 4: assume well mixed, steady state: accumulation = 0, sterile feed What does the general mass balance equation look like around a fermenter? Lecture 4 What does the dilution factor physically represent? Lecture 4: 1/D = the time it takes to replace one volume of liquid in the fermenter. What are the two main advantages of chemostat with recycle? Lecture 5 increase productivity, stability List three factors that affect K L a Explain the moelcular basis of the Crabtree effect. Plot μ vs S that shows what happens when the crabtree effect occurs. Review problems in Homework 2 and 3 per email requsts Review problems that solve for S and X instead of the monod constants as most of the homework has done.

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4) A strain of mold was grown in a batch culture on glucose and the following data were obtained: Time (hr) Cell Concentration (g/l) Glucose conc.(g/l) 0 1.25 100 9 2.45 97 16 5.1 90.4 23 10.5 76.9 30 22 48.1 34 33 20.6 36 37.5 9.38 40 41 0.6

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The production of a certain product has been described by the following expression: R p where R p is the specific productivity (mg product/g cell/hr), m is the specific growth rate (hr - 1 ), and A and B are constants equal to 1.0 mg product/ g cells and 1.0 mg product/g cells/hr, respectively. Calculate the steady state cell, substrate and product concentration in a chemostat. Assume that the Monod kinetics apply and the substrate utilization for maintenance and product synthesis is negligible. max =2.0 hr -1 K s = 0.001 g /l Y x/s = 0.5 g cells/g substrate F= 1.0 l/hr V=1.0 l S in = 10 g/l (substrate inlet conc.)

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A) Calculate the sterilization time for a 10 L fermentor assuming a B. stearothermophilus spore count of 10E(6)/ml for the media and a probability of contamination of 10E(-3). The sterilization will be conducted at 121°C. B)
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Problem solving - Lecture 9 CHE/BME339 Take a break Review...

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