Final Exam 2003 - FINAL EXAM CHE 339/BIO 335/BME 339 FALL...

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FINAL EXAM CHE 339/BIO 335/BME 339 FALL 2003 9:00-12:00 NOON Wednesday, December 10, 2003 CPE 2.220 YOUR NAME _________________________________ 1) The gene fraternity is thought to be responsible for a disorder characterized by complete lapse of mental function in young adult males, particularly following moderate alcohol consumption. This gene is present in about 40% of the population. Describe in some detail three DNA diagnostic methods for detecting the presence or absence of this gene (one of the methods you need to describe must be PCR/OLA (PCR/oligonucleotide ligarion)). (8) 2) (a) Draw a schematic of the structure of IgG antibodies showing key relevant regions (2). (b) Describe and explain the rational of the key steps in the isolation of hybridoma cells that produce monoclonal antibodies for a specific compound (6). (c) What is a human-mouse chimeric monoclonal antibody and why is it useful? (2) (d) In recent years it has been possible top isolate antibodies from libraries expressed in E.coli . Briefly describe how that is done (4).
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3) The generation of engineered proteins can be accomplished by one of three means: (a) site specific mutagenesis whereby a sequence encoding a particular amino acid is changed (b) random mutagenesis of an entire gene (followed by screening of a library to isolate a protein with improved function) and (c) DNA shuffling or invitro recombination. Describe a method for (a) and (b) for 2 points each. 2 BONUS point for (c) (total of 4 points+ 2 BONUS points). 4) The protein apoptin has just been purified and shown to be a potent inducer of apoptosis in mammalian cells. Because apoptin is isolated from human tissues the entire world suply is only 10 μg. This amount is too small to allow the production of apoptin specific antibodies. You are asked to clone the apoptin gene. Describe how you would attempt to do that (5). 5) You have been asked to teach your fellow student Joe Aggie how to do a Southern hybridization
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This note was uploaded on 12/13/2011 for the course BME 339 taught by Professor Georgiou during the Spring '11 term at University of Texas at Austin.

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Final Exam 2003 - FINAL EXAM CHE 339/BIO 335/BME 339 FALL...

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