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Final Exam 2004 - FINAL EXAM CHE 339/BIO 335 FALL 2004...

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FINAL EXAM CHE 339/BIO 335 FALL 2004 2:00-5:00 pm Saturday, December 11,2004 YOUR NAME _________________________________ 1) A. Derive expressions for the biomass concentration, X, and the substrate concentration, S, in the outflow of a continuous fermenter, assuming that the cells obey ideal Monod kinetics. Draw a plot showing how the biomass and substrate concentrations vary as a function of the Dilution rate D. (3) B. In some cases it is advantageous to use two stirred tanks in series, i.e. the outflow from the first tank is the feed to the second tank which has a different volume (V2) than the first tank. Derive an expression relating the dilution rate to the growth rate in the second tank . (5) C. Name two advantages and two disadvantages of continuous fermentations (2) 2) The following questions relate to mammalian cell cultivation; Answer in two sentence or less each. (a) What are transformed and what are primary cells (2) (b) Why is CO 2 used in mammalian cell culture (2)?
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