Final Exam 2004 - FINAL EXAM CHE 339/BIO 335 FALL 2004...

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FINAL EXAM CHE 339/BIO 335 FALL 2004 2:00-5:00 pm Saturday, December 11,2004 YOUR NAME _________________________________ 1) A. Derive expressions for the biomass concentration, X, and the substrate concentration, S, in the outflow of a continuous fermenter, assuming that the cells obey ideal Monod kinetics. Draw a plot showing how the biomass and substrate concentrations vary as a function of the Dilution rate D. (3) B. In some cases it is advantageous to use two stirred tanks in series, i.e. the outflow from the first tank is the feed to the second tank which has a different volume (V2) than the first tank. Derive an expression relating the dilution rate to the growth rate in the second tank . (5) C. Name two advantages and two disadvantages of continuous fermentations (2) 2) The following questions relate to mammalian cell cultivation; Answer in two sentence or less each. (a) What are transformed and what are primary cells (2) (b) Why is CO 2 used in mammalian cell culture (2)?
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(c) Name three reasons why Fetal Calf Serum is used in mammalian cell culture AND also its main drawback(2) (d) Why are mammalian cells used for the production of recombinant proteins and what are the three major limitations in their use? (2) (e) One of the complications in mammalian cell cultivation is supplying enough oxygen to the cells. Explain why this is a problem and how it is addressed (2) 3) Gene X is 1200 bp and it is flanked on both sides by the sequence 5’-GTTAAC-3’, the recognition
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This note was uploaded on 12/13/2011 for the course BME 339 taught by Professor Georgiou during the Spring '11 term at University of Texas at Austin.

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Final Exam 2004 - FINAL EXAM CHE 339/BIO 335 FALL 2004...

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