Final exam 2005_solutions

Final exam 2005_solutions - FINAL EXAM CHE 339/BIO 335/BME...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
FINAL EXAM CHE 339/BIO 335/BME 339 FALL 2005 Thursday, December 15, 2005 YOUR NAME _________________________________ 1. The mutant form of the gene fraternity is thought to be responsible for a disorder characterized by complete lapse of mental function in young adult males, particularly following moderate alcohol consumption. This mutation is a 10 base deletion and it is present in about 40% of the population. Describe in some detail two DNA diagnostic methods for detecting the presence or absence of this gene (one of the methods you need to describe must be PCR/OLA (PCR/oligonucleotide ligarion)). 5 points
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
(a) Draw a schematic of the structure of IgG antibodies showing key relevant regions (2). (b) Describe and explain the rational of the key steps in the isolation of hybridoma cells that produce monoclonal antibodies for a specific compound (4). (c) What is a human-mouse chimeric monoclonal antibody and why is it useful? (2) (d) In recent years there has been strong interest in isolating completely human antibodies using bacteria and filamentous phages. How is this done (4) 12 points total 3. The generation of engineered proteins can be accomplished by one of three means: (a) site specific mutagenesis whereby a sequence encoding a particular amino acid is changed (b) random mutagenesis of an entire gene (followed by screening of a library to isolate a protein with improved function) and (c) DNA shuffling or in vitro recombination. 1. Briefly describe each method (2 each) 2. What is accomplished by DNA shuffling; i.e. why we use DNA shuffling for portin engineering? (1 point) 7 points total
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 10

Final exam 2005_solutions - FINAL EXAM CHE 339/BIO 335/BME...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online