Final exam 2005 - FINAL EXAM CHE 339/BIO 335/BME 339 FALL...

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FINAL EXAM CHE 339/BIO 335/BME 339 FALL 2005 Thursday, December 15, 2005 YOUR NAME _________________________________ 1. The mutant form of the gene fraternity is thought to be responsible for a disorder characterized by complete lapse of mental function in young adult males, particularly following moderate alcohol consumption. This mutation is a 10 base deletion and it is present in about 40% of the population. Describe in some detail two DNA diagnostic methods for detecting the presence or absence of this gene (one of the methods you need to describe must be PCR/OLA (PCR/oligonucleotide ligarion)). 5 points 2. (a) Draw a schematic of the structure of IgG antibodies showing key relevant regions (2). (b) Describe and explain the rational of the key steps in the isolation of hybridoma cells that produce monoclonal antibodies for a specific compound (4). (c) What is a human-mouse chimeric monoclonal antibody and why is it useful? (2) (d) In recent years there has been strong interest in isolating completely human antibodies using bacteria and filamentous phages. How is this done (4) 12 points total
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3. The generation of engineered proteins can be accomplished by one of three means: (a) site specific mutagenesis whereby a sequence encoding a particular amino acid is changed (b) random mutagenesis of an entire gene (followed by screening of a library to isolate a protein with improved function) and (c) DNA shuffling or in vitro recombination. 1. Briefly describe each method (2 each) 2. What is accomplished by DNA shuffling; i.e. why we use DNA shuffling for portin engineering? (1 point) 7 points total 4. (a) What are the two main methods for introducing genes into human cells for gene therapy purposes? (1 point) (b) list two desirable and two undesirable features of adenoviruses for gene therapy (2 points) 3 points total 5. The gene for the mammalian protein Argonaut has been cloned in
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Final exam 2005 - FINAL EXAM CHE 339/BIO 335/BME 339 FALL...

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