339 HW 5 - BME 339/BIO 335/CHE 339 Fall 2010 Homework #5...

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BME 339/BIO 335/CHE 339 Fall 2010 Homework #5 Assigned: October 26th, 2010 Due: Thursday, November 4th, 2010 1. The enzyme NcoI recognizes the sequence 5' -C^C A T G G-3’ 3' -G G T A C^C-5' It is used to cut the genome of a bacterium whose genome consists of 65% G or C and 35% A or T. The genome of the bacterium is 2,800,000 bp. How many fragments would you estimate to be generated by digestion of the genomic DNA with NcoI? 2. Draw a restriction map for a circular DNA molecule using the following data: Restriction enzyme Fragment Size EcoRI 1200, 1100, 800, 400 HindIII 2500, 1000 BamHI 3500 EcoRI+HindIII 1200, 800, 700, 400, 300, 100 HindIII+BamHI 1800, 1000, 700 EcoRI+BamHI 1200, 1100, 500, 400, 300 3. A linear DNA fragment is radiolabelled at one end with 32 P. Partial digestion of this DNA with EcoRI (THIS MEANS INCOMPLETE REACTION HENCE ALL THE POSSIBLE FRAGMENTS ARE GENERATED) followed by agarose gel electrophoresis and autoradiography showed the formation of DNA fragments of sizes
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This note was uploaded on 12/13/2011 for the course BME 339 taught by Professor Georgiou during the Spring '11 term at University of Texas at Austin.

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339 HW 5 - BME 339/BIO 335/CHE 339 Fall 2010 Homework #5...

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