{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

HW3_2011_sol

# HW3_2011_sol - Problem 1 Problem 2 a Based on steady state...

This preview shows pages 1–3. Sign up to view the full content.

Problem 1 Problem 2 a) Based on steady state cell mass balance, it can be shown that the dilution rate F/V (D) must equal specific growth rate (μ). Therefore, up to certain maximum D, incremental increase of D can increase specific growth rate of the culture leading to increase of the cell density. b) Since this is a substrate limited growth, at steady state, assume there is no substrate at output stream. Then, Y X/S = X / (0.05 × 180 – 0). By plotting 1/D vs 1/Y X/S , we can find Y TRUE at the Y-intercept. 1/Y X/S = m/D + 1/Y TRUE D (hr-1) X (g/L) Ideal Yx/s 1/D 1/Yx/S 0.05 1.58 2.86 0.175555556 20 5.696203 0.1 2.17 2.86 0.241111111 10 4.147465 0.15 2.47 2.86 0.274444444 6.666667 3.643725

This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document
0.2 2.65 2.86 0.294444444 5 3.396226 0.25 2.76 2.86 0.306666667 4 3.26087 0.3 2.83 2.86 0.314444444 3.333333 3.180212 0.35 2.86 2.86 0.317777778 2.857143 3.146853 0.4 2.84 2.84 0.315555556 2.5 3.169014 0.45 2.74 2.74 0.304444444 2.222222 3.284672 0.5 2.25 2.25 0.25 2 4 0.52 1.32 1.32 0.146666667 1.923077 6.818182 0.53 0 0 0 1.886792 #DIV/0! Using the greyed area data points to plot, Then fit to linear eqn, Y TRUE = 1/2.647 = 0.378 (g cell/ g substrates)
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

### What students are saying

• As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

Kiran Temple University Fox School of Business ‘17, Course Hero Intern

• I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

Dana University of Pennsylvania ‘17, Course Hero Intern

• The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

Jill Tulane University ‘16, Course Hero Intern