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Final+lecture - Course Highlights Photon has...

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Course Highlights
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Photon has energy (electron volts)= E E = h · c/ λ h = Planck’s constant = 4.14x10 -15 eV sec C = speed of light = 3x10 10 cm/sec* λ = wavelength = cm** * also expressed as M/sec ** can also be expressed as nm, or µm
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What is the importance of eV ?
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Type of bond s: Dissociation energy for molecular (atom) bonds
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Eye (cornea ) Eye (retina) Cancer, OCT ,DOS Cell Trapping General Surgery Bond-breaking threshold Typical lasers: use in biomedicine , wavelength, & photon energy ultraviolet visible infrared
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Photophysics in spot irradiance = power density = W/cm 2 [From: Berns, M.(1998) Sci. Am.  198:62-7]   Jablonski Diagram (linear single photon absorption) chemistry Vibrational heat
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MOMENTUM TRANSFER Multi-photon Single photon
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   Laser Scissors & Tweezers Berns  M., Scientific American 278: 68, 1998   TWEEZERS SCISSORS SCISSORS TWEEZERS DNA not quite reality
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What happens when light (photons) comes in contact with cells?
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Photophysics in spot irradiance = power density = W/cm 2 [From: Berns, M.(1998) Sci. Am.  198:62-7]   Jablonski Diagram (linear single photon absorption) chemistry Vibrational heat
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Solar Sail Example of Radiation Pressure IKAROS (ICARUS) Science Fiction: 1990’s (Greek Mythology) 2010: Japan solar sail in orbit
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[ + - = 1 sin T Rsin2θ c P n Fgrad 2 1 Use the below equation in determining force . w ( Q ) n = 1.33 (refractive index of medium) Q = 0.12 (dimensionless geometric factor,0.12) C = 3x10 8 m/sec orce
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From: Berns and Greulich, pg 742 (appendix) e
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Sperm: highly specialized cell flagellum = microtubule driven motility packed with mitochondria Flagellum Size = 0.1 – 1 µm 25,000 – 50,000 X magnification cristae cross -section 9+ 2 microtubules
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outer membrane inner membrane the more H + the more ATP
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Effects of CCCP on mitochondrial membrane potential. The ratio value red/green fluorescence is plotted against time in seconds. Ratio values are measured over a 10-s interval for test sperm group with CCCP, in magenta and control sperm group without CCCP, in black. Each track represents an individual sperm. Hit the sperm with cyanide : carbonyl cyanide 3-chlorophenylhydrazone (CCCP)
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Sperm Competition “sperm races”
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