{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lecture-6-kscN152 - Developmental Neurobiology N152 Karina...

Info icon This preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
10/10/11 1 Karina S. Cramer [email protected] 10/11/11 Developmental Neurobiology N152 Neuronal Migration – key points • Neural crest migration gives rise to the peripheral nervous system and has segment specificity • In the CNS, cells undergo migration at the ventricular zone during a process called interkinetic nuclear migration
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
10/10/11 2 Neuronal Migration – key points New neurons migrate along radial glial cells as they move toward the cortical plate. Neuronal Migration – key points Radial glia are progenitors for neurons and glia
Image of page 2
10/10/11 3 Neuronal Migration – key points Migration along glial cells occurs radially and tangentially Migration along glial cells happens in many areas of developing nervous system. Neuronal Migration – key points Migration is seen in several secondary zones of neurogenesis (i.e., neurogenesis in cells that have moved away from the ventricular zone) These areas have been found to have some limited neurogenesis in adults External granule cell layer of cerebellum Subventricular zone: wall of lateral ventricle Hippocampus Adult retina in amphibians
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
10/10/11 4 Outline: Neuronal fate specification • Cell fate specification: further interactions that lead to formation of distinct cell types. • Tools for studying this process include genetics, transplantation, and co-cultures. • Mosaic development (as in C. elegans) occurs when different neurons derive from different precursor with very limited potential. • Patterns of gene expression along the axes (see lecture 2) lead to changes in gene expression that influence cell fate. • Timing matters: different factors change their expression over time, so that progeny have different fates depending on when they were born. • Cell-cell interactions provide signals that influence cell fate. • Some progenitors in vertebrates have multiple possible fates, and the factors that determine several of their choices have been identified. Mutational analysis Forward genetics : Identify new genes that have effects on neural differentiation Reverse genetics : molecular engineering to knock out or over express genes that are candidates for roles in neuronal fate determination or differentiation Testing Cell Fate
Image of page 4
10/10/11 5 Figure 22-7 Molecular Biology of the Cell (© Garland Science 2008) Tissue transplants to examine whether a cell s fate is determined Figure 22-7 Molecular Biology of the Cell (© Garland Science 2008) Tissue transplants to examine timing of determination • Transplantation studies can also be used to ascertain when cell fate determination occurs. Heterochronic transplants to a homologous location can be used to test competence at different times. • Depends on when factors are released, and when cells are competent to respond to factors.
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
10/10/11 6 Exogenous factors Localized signals Transplant cells tissues Transplant analysis (combining genetics with transplant) Can reveal whether neuronal phenotypes are extrinsically regulated by the gene in
Image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern