Worksheet+12 - Bio 93 Worksheet 12 Lecture 23 and 24 Pooja...

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Bio 93 Pooja Patel Worksheet 12 – Lecture 23 and 24 [email protected] Lecture 23: Recombinant DNA Technology Type 1 Diabetes o Inheritance ________________ disease _________________________________ influence expression o Symptoms High blood glucose to defective insulin production o Treatment Insulin purified from pigs ________________: synthetic human insulin Produced by recombinant technology More effective Fewer side effects DNA Cloning in Bacteria o Ability to combine ________________ from different sources into a single ____________________ 1. Make recombinant DNA a. Cut DNA with ______________________________ a.i. Use same restriction enzyme to get same cut b. Ligate DNA with ________________ b.i. DNA will join by base pairing
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Bio 93 Pooja Patel Worksheet 12 – Lecture 23 and 24 [email protected] 2. ________________ bacterium 3. _____________________ and ______________________ a. Introduce DNA into bacterial cells with mutation on recombinant gene b. Plate bacteria on agar plate and let colonies grow DNA Amplification
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This note was uploaded on 12/13/2011 for the course BIOSCI 93 taught by Professor Staff during the Fall '11 term at UC Irvine.

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Worksheet+12 - Bio 93 Worksheet 12 Lecture 23 and 24 Pooja...

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