Worksheet+12+-+Answer+KEy

Worksheet+12+-+Answer+KEy - Bio 93 Worksheet 12 Lecture 23...

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Bio 93 Pooja Patel Worksheet 12 – Lecture 23 and 24 patelph@uci.edu Lecture 23: Recombinant DNA Technology Type 1 Diabetes o Inheritance Polygenic disease Environmental factors influence expression o Symptoms High blood glucose to defective insulin production o Treatment Insulin purified from pigs Humulin: synthetic human insulin Produced by recombinant technology More effective Fewer side effects DNA Cloning in Bacteria o Ability to combine genes from different sources into a single DNA molecule 1. Make recombinant DNA a. Cut DNA with restricted enzymes a.i. Use same restriction enzyme to get same cut b. Ligate DNA with DNA ligase b.i. DNA will join by base pairing 2. Transform bacterium 3. Amplification and Selection
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Bio 93 Pooja Patel Worksheet 12 – Lecture 23 and 24 patelph@uci.edu a. Introduce DNA into bacterial cells with mutation on recombinant gene b. Plate bacteria on agar plate and let colonies grow DNA Amplification o Polymerase Chain Reaction (PCR)
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This note was uploaded on 12/13/2011 for the course BIOSCI 93 taught by Professor Staff during the Fall '11 term at UC Irvine.

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Worksheet+12+-+Answer+KEy - Bio 93 Worksheet 12 Lecture 23...

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