fingerPrintingSlides

fingerPrintingSlides - 1 Tracking genes in families and...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 1 Tracking genes in families and populations DNA markers are co-dominant 2 general types of DNA Markers Single base changes SNPs Single Nucleotide Polymorphisms(can be detected by: RFLPs ASOs Allele Specific Oligonucleotides microarrays Sequencing Changes in repeat number HVRs HyperVariable Regions Inheritance animals have 2 copies of each chromosome. Sperm and eggs carry one copy of each. Offspring get 1 copy from mom & 1 from dad. There are always 2 DNA markers for each locus, even if the bands are the same size B B b b B b b B B B B B Sperm carry either Black or white Eggs carry either the Black or the white RFLP = Restriction Fragment Length Polymorphism 3.5 3.5 1.5/2.0 1.5/2.0 3.5 1.5/2.0 1.5 kb 2.0 kb 3.5kb GAATTC 1.5 kb 2.0 kb GGATTC 3.5 kb RFLPs = a binary code. a binary code. Yes or no - cut or not cut GAATTC or not 1,1 2,2 1,2 1,2 1,1 2,2 1,2 2 1.5 3.5 general population (unrelated) 1,2 1,1 2,2 2,2 1,1 1,1 1,2 mutation occurs in this person ? ? ? ORIGIN OF AN ASSOCIATION BETWEEN AN RFLP & A DOMINANT MUTATION ? Al ele 1 = 3.5 kb Al ele 2 = 2 & 1.5 kb 1,1 2,2 1,2 1,2 1,1 2,2 1,2 2 1.5 3.5 general population (unrelated) 1,2 1,1 2,2 2,2 1,1 1,1 1,2 mutation occurs in this person ? ? ? ORIGIN OF AN ASSOCIATION BETWEEN AN RFLP & A DOMINANT MUTATION ? Affected?? Y N Y N Y N ? probability of affected = 1 0 1/2 Affected but does not carry the RFLP original y linked to disease in this family. Must be a cross over! ASOs = Allele Specific Oligonucleotides How to detect s that does not change Restriction site?- Normal = CTCCTGAGGAGAAGTCTGC = 19mer- Mutant = ----------A----------------------------Label the oligo.-Anneal @1M NaCl = 2/AT & 3/GC pair-11GC x 3 = 33 & 8AT x 2 = 16 33+16 = 49-Mismatch Tm = <46 2 SNP mapping 1. Single nucleotide polymorphisms(SNPs -pronounced "snips") are DNA sequence variations that occur when a single nucleotide (A,T,C,or G) in the genome sequence is altered. For example a SNP might change the DNA sequence A A GGCTAA to A T GGCTAA. 2. For a variation to be considered a SNP, it must occur in at least 1% of the population....
View Full Document

This note was uploaded on 12/13/2011 for the course BIOSCI 137 taught by Professor Staff during the Fall '11 term at UC Irvine.

Page1 / 5

fingerPrintingSlides - 1 Tracking genes in families and...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online