globin_gene_struct_++08

globin_gene_struct_++08 - Globin and gene structure...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Globin and gene structure 11/13/08 J.L. Marsh 137B - 1 - GLOBIN GENES AND GENE STRUCTURE Objectives: Gene structure, Consensus & conserved sequences (the beta globin gene) (Efstradiatis paper 1 1.Anatomy of a gene 1 2 3 4 5 6 7 8 ! AUG TAA TAG TGA [GT AG] cap(ppp7CH3Gp) AATAAA AAAAAAn(1-200) AAAAn TATA Termination? LETS COMPARE SEQUENCE OF cDNA (mRNA) AND GENOMIC DNA -mRNA 1] PROTEIN CODING REGION recognized as a single LONG OPEN READING FRAME = PRIMARY MEANS OF RECOGNIZING CODING SEQUENCE -Met = start translation codon. -TAG, TGA or TAA = stop translation codon - Code is same a bacteria 2] UNTRANSLATED PARTS AT 5' AND 3' ENDs - 5' Untranslated = translation initiation - 3' Untranslated = ? (mRNA localization) They are part of the exons 3] 3 MODIFICATIONS occur to the primary RNA transcript. 1] RNA IS MODIFIED AT 5' END = CAPPED Ribosomes scan Cap = m7G(5')ppp(5')N---- Thought to be necessary for translation Occurs shortly after initiation 2 It is catalyzed by guanylyl and methyl transferases which are nuclear enzymes Most animal viruses that replicate in the cytoplasm contain similar activities and cap their mRNAs Cap binding proteins facilitate initiation complex formation 1 Eftstratiadis et al Cell 21:653-663 (1980) The structure and evolution of the Human B-globin family 2 Shatkin Cell 40:223-224 (1985) mRNA cap binding proteins: Essential factors for initiating translation
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Globin and gene structure 11/13/08 J.L. Marsh 137B - 2 - [Shatkin diagram] 2] RNA IS MODIFIED @ 3' END - POLY A Poly A is added by terminal transferase This requires cleavage of mRNA and addition of AAAn
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 12/13/2011 for the course BIOSCI 137 taught by Professor Staff during the Fall '11 term at UC Irvine.

Page1 / 6

globin_gene_struct_++08 - Globin and gene structure...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online