high-throughput

high-throughput - Microarrays, RNA-Seq and Other...

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Microarrays, RNA-Seq and Other High-Throughput Methods BMI/CS 576 www.biostat.wisc.edu/bmi576/ Mark Craven craven@biostat.wisc.edu Fall 2011 Measuring RNA abundances what is varied: individuals, strains, cell types, environmental conditions, disease states, etc. what is measured: RNA quantities for thousands of genes, exons or other transcribed sequences genes mRNAs
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Gene-expression profiles for yeast cell cycle rows represent yeast genes columns represent time points as yeast goes through cell cycle color represents expression level relative to baseline (red=high, green=low, black=baseline) Gene-expression profiles for leukemia patients rows represent genes columns represent people with 2 subtypes of leukemia: ALL and AML
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Gene-expression profiles for ovarian cancer samples Gillet et al., PNAS 2011 Gene-expression profiles for genes that induce differentiation Ivanova et al., Nature 2006
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Measuring protein and metabolite abundances in a similar manner, we can characterize cells in terms of protein or metabolite (small molecule) abundances this is not as common as mRNA profiling, however, because the technology for doing it is not as mature Measuring protein and metabolite abundances varied : individuals, strains, cell types, environmental conditions, disease states, etc. measured : RNA quantities technology: microarrays varied : same as above measured : protein quantities technology: 2D gel electrophoresis + mass spec varied : same as above measured : small molecule quantities technology: 2D gel electrophoresis + mass spec
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This note was uploaded on 12/15/2011 for the course BMI 576 taught by Professor Staff during the Fall '11 term at Wisc Green Bay.

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high-throughput - Microarrays, RNA-Seq and Other...

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