AandP 2 Lab 2 Lecture

AandP 2 Lab 2 Lecture - Biology 112 Lab 2 Hematology and...

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1 Part I: Hematology Lab Tasks: • Total counts - RBC’s (be able to explain procedure & applications for total WBC counts) • Hematocrit & Hemoglobin content Biology 112 Lab 2: Hematology and Physical Transport Total Blood Cell Counts: The Hemacytometer Microscopic grid lines etched in the mirrored surfaces create chambers of precise volume. By counting the number of cells and multiplying by a known factor, an accurate count of cells / unit volume can be made.
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2 Loading the Hemacytometer Hemocytometer Grid Total Counts - Red Blood Cells Count all RBC’s from all “R” areas of the grid. Multiply your count by 10,000 Your calculations yield the number of RBC’s per cubic millimeter of blood. 100x 1,000x
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