AandP 2 Lab 2 Lecture

AandP 2 Lab 2 Lecture - Biology 112 Lab 2: Hematology and...

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1 Part I: Hematology Lab Tasks: • Total counts - RBC’s (be able to explain Biology 112 Lab 2: Hematology and Physical Transport Total Blood Cell Counts: The Hemacytometer Microscopic grid lines etched in the mirrored surfaces create chambers of precise volume. By counting the number of cells and multiplying by a known factor, an accurate count of cells / unit volume can be made.
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2 Loading the Hemacytometer Hemocytometer Grid Total Counts - Red Blood Cells Count all RBC’s from all “R” areas of the grid. Multiply your count by 10,000 Your calculations yield the number of RBC’s per cubic millimeter of blood. 100x 1,000x
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3 What if a cell falls on a grid line? If it falls on the left or top line of the square, count it as part of that square. If it falls on the right or bottom line, do not count it as part of that square - it will get counted in one of the next squares. .. Hematocrit - packed volume of red cells Draw line around capillary tube 1.5 cm. from end Bring blood up to top of capillary tube using disposable pipette Cap end of capillary tube with your finger, and touch tip to crumpled paper towel. Carefully roll your finger off of top of capillary
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AandP 2 Lab 2 Lecture - Biology 112 Lab 2: Hematology and...

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