536lecntwtbnk_5 11.45.23 PM

536lecntwtbnk_5 11.45.23 PM - MIT OpenCourseWare...

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MIT OpenCourseWare http://ocw.mit.edu 5.36 Biochemistry Laboratory Spring 2009 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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________________________________________________________________________________ _______________________________________________________________________________ 5.36 Lecture Summary #5 (shortened) Thursday, March 5, 2009 CI-M assignments : The first draft of your mini-review is due today. Prior to the March 10 th CI-M lecture please read: Gorre, M. E. et al. Clinical Resistance to STI-571 (STI-761 = Gleevec) Cancer Therapy Caused by BCR-ABL Gene Mutation or Amplification. Science 293 , 876-880 (2001). The article is posted on steller. Next Laboratory Session: #9 Topic: Site-Directed Mutagenesis and Transformation Overview of what you’ve completed in Module 4 and what will come in Module 5: Module 4 (Sessions 1-8) Expressed H396P Abl(229-511) in BL21-DE3 expression cells Purified, dialyzed, concentrated, and quantified your expressed protein Visualized the purified H396P Abl(229-511) by SDS-PAGE gel analysis Isolated wt Abl kinase domain vector DNA for mutagenesis in Module 5 (and checked for the expected insert by restriction digestion)
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This note was uploaded on 12/16/2011 for the course BIOLOGY 101 taught by Professor Mr.wallace during the Fall '11 term at Montgomery College.

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536lecntwtbnk_5 11.45.23 PM - MIT OpenCourseWare...

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