mock60 - Historical Context of Vitamin B12 Pernicious...

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Historical Context of Vitamin B 12 Pernicious anemia Megaloblastic anemia Neuropathy: particularly degeneration of spinal cord Universally fatal Extrinsic factor from liver Patients were not producing enough Gastric acid to denature R protein Intrinsic factor
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Structure of Vitamin B 12 Cobalamins Corrin ring contains central cobalt atom Adenosylcobalamin Methylcobalamin
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12 Receptors on ileal mucosal cells Transcobalamin II Portal circulation Receptor on the cell surface Endocytosis and lysosomal degradation of the complex Reduction of cobalt Cytosolic methylation Mitochondrial adenosylation
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Role of Ascorbate in the Degradation of Tyrosine Oxidation of p-hydroxyphenylpyruvate Maintain Cu 2+ ? Homogentisate oxidation Maintain Fe 2+ ? Synthesis of epinephrine Bile acid synthesis (7 α -hydroxylase) Enhancement of iron absorption Antioxidant
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Incubation 2: Transfer to a microtiter well coated with biotin-BSA ( ) Incubation 1: Biotin incubated with HRP-avidin Detection of Biotin ( ) by HRP-Avidin ( ) B B B B B B B B B B B B B B B B B B B B B B B B B Transfer
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Standard Curves for Biotin 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 0 300 600 900 1,200 1,500 1,800 Absorbance Avidin-binding Substance (pmol/L)
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Biotin Catabolites Bisnorbiotin O C S HC H 2 C HN CH CH NH
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mock60 - Historical Context of Vitamin B12 Pernicious...

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