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Unformatted text preview: MIT OpenCourseWare http://ocw.mit.edu 5.36 Biochemistry Laboratory Spring 2009 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms . URIECA Biochemistry I. Scientifc background A. Review oF kinases and phosphatases B. Abl kinase and Bcr-Abl C. Kinase inhibitors as cancer drug targets II. Overview oF labs A Review of Kinases A kinase is an enzyme that catalyzes the transfer of a phosphate group from ATP onto a substrate (target molecule). NH 2 O- O N N P OH O O- N O O O N kinase + - O P O P O P O O H H O- O- O- H H OH H ATP ADP A phosphatase is an enzyme that catalyzes the removal of a phosphate group from a target molecule: the reverse reaction. O- P O OH phosphatase O O- substrate substrate substrate substrate A Review of Kinases A kinase is an enzyme that catalyzes the transfer of a phosphate group from ATP onto a substrate (target molecule). NH 2 O- O N N P OH O O- N O O O N kinase + - O P O P O P O O H H O- O- O- H H OH H ATP ADP A phosphatase is an enzyme that catalyzes the removal of a phosphate group from a target molecule: the reverse reaction. O- P O OH phosphatase O O- substrate substrate substrate substrate Protein kinases modify other proteins Serine/threonine-kinases phosphorylate target proteins on a Ser or Thr residue. OH OH OH O O O H 3 N H 3 N H 3 N O O O Ser, S ____, T Thr Tyr, ___ Y Tyrosine-kinases phosphorylate target proteins on a Tyr residue. Biological Relevance of Protein Kinases Kinases (and phosphatases) can regulate the activity of a protein in several ways: For example, phosphorylation of a protein target can • create new binding site(s) for that protein. signaling pathway P P kinase interaction with is activated OR additional signaling phosphatase inhibited molecules Biological Relevance of Protein Kinases Kinases (and phosphatases) can regulate the activity of a protein in several ways: For example, phosphorylation of a protein target can • create new binding site(s) for that protein. • result in conformational changes that affect binding… Biological Relevance of Protein Kinases Kinases (and phosphatases) can regulate the activity of a protein in several ways: For example, phosphorylation of a protein target can • create new binding site(s) for that protein. • result in conformational changes that affect binding… kinase signaling pathway interaction with is activated OR phosphatase additional signaling inhibited molecules P P or modulate enzyme activity . kinase inactive active form form phosphatase P kinase inactive active form form phosphatase P The phosphorylated form of an enzyme may be the active or the inactive form. kinase active P inactive form form phosphatase Image courtesy of Mike Jones Phosphorylation by kinases can “switch on” an enzyme substrate to produce signifcant quantities oF active enzyme on timescale oF seconds to minutes ....
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This note was uploaded on 12/16/2011 for the course BIOLOGY 101 taught by Professor Mr.wallace during the Fall '11 term at Montgomery College.

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Slides1 11.45.23 PM - MIT OpenCourseWare http://ocw.mit.edu...

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