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Strawberry DNA Extraction Lab Packet

Strawberry DNA Extraction Lab Packet - Module 3 Strawberry...

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Rev. 2/18/04 ! 2003, Pioneer Hi-Bred International, Inc. Page 1 of 11 Educational Services Program (ESP) Phone: 515-270-3364 or 3175 Module 3: Strawberry DNA Extraction Teacher/Leader Target Audience: 7-12 Life Science, Biology, Ag Science Overview: In this lab, students will extract DNA from a strawberry using everyday materials and observe its physical appearance. Objectives: As a result of participating in this activity, students will: Know how to extract DNA from strawberries. Observe what DNA looks like to the naked eye. Learn that DNA is found in every living and once living thing. Understand that DNA is found in all the food we eat. National Science Education Standards: The following information was obtained from the National Science Education Standards . National Research Council, copyright 1996, National Academy Press. Content Standard C: Life Science 5-8 Reproduction and Heredity. Every organism requires a set of instructions for specifying its traits. Heredity is the passage of these instructions from one generation to another. Content Standard C: Life Science 9-12 The Cell. Cells store and use information to guide their functions. The genetic information stored in DNA is used to direct the synthesis of the thousands of proteins that each cell requires. The Molecular Basis of Heredity . In all organisms, the instructions for specifying the characteristics of the organism are carried in DNA, a large polymer formed from subunits of four kinds (A, G, C, and T). The chemical and structural properties of DNA explain how the genetic information that underlies heredity is both encoded in genes (as a string of molecular “letters”) and replicated (by a templating mechanism). Each DNA molecule in a cell forms a single chromosome. Science Content: To show students a method to extract DNA from strawberries. Science Process Skills: Observing Communicating Inferring Life Skills: Communicating Learning Collaboration Time: 50 minutes
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Rev. 2/18/04 ! 2003, Pioneer Hi-Bred International, Inc. Page 2 of 11 Educational Services Program (ESP) Phone: 515-270-3364 or 3175 Materials: Worksheets: Student pre-lab, DNA extraction lab, student response. 1 Zip-type, freezer bag (6”x 9”) 1 Coffee filter, cone-shaped, #2 size 1 Plastic cup, 5 oz. 1 Plastic pipette 1 Strawberry 10 mLs DNA extraction buffer (soapy, salty water) 15 mLs ice cold ethanol in test tube Lab Preparations: The following solutions should be prepared by the instructor in advance of the lab. DNA extraction buffer Materials: 50 mLs of a clear hair shampoo with EDTA (Ex. Suave). Do not use one that contains a conditioner. 1 tsp of NaCl (table salt) 450 mLs water In a one-quart jar or beaker, gently mix the materials so as not to create a lot of bubbles. This will make enough extraction buffer for 50 groups of two students. Provide pipettes for students to draw 10 mLs of the extraction buffer from the jar to add to their plastic bags with mashed strawberries in step three of part one.
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