Lecture7 - Lecture 7. Epigenetic reprogramming Lisheng WANG...

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Lecture 7. Epigenetic reprogramming Lisheng WANG lwang@uottawa.ca Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa
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1. Epigenetic reprogramming of somatic cells a. Somatic cell nuclear and Chromosome transfer b. Cell fusion c. Cell extract d. Culture-induced reprogramming e. Genetic reprogramming 2. Parthenogenesis 3. hybrid embryos – a potential approach to generate organs for transplantation 4. From adult cells to adult stem cells from progenitors to adult stem cells from committed one lineage to another lineage from one type to another type of stem cells Epigenetic reprogramming
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1. Methods for Epigenetic Reprogramming of Somatic Cells ES b. Cell fusion c. Cell extract d. Culture induced a. Nuclear/ chromosome transfer e. Genetic reprogramming Oct4+cMyc +Klf4+Sox2 (Cell 2006;126:677) Somatic cells Nature. 2010 Jun 10; 465:704-712 (review).
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a. Somatic cell nuclear transfer (SCNT) reprogramming ES, NSC, T, B cells, granulocytes, etc (Cell 2006;126:652) Unsuccessful for hESC
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a. Functional assays of pluripotency (after reprogramming) Amy Ralston and Janet Rossant. Reproduction (2010) 139 35-44 (Review) D. Demethylation analysis
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a. SCNT reprogramming – cloning timeline (Cell 2006;126:652) Nature 2007; 445 : 800-801 Rat
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a. Epigenetic reprogramming of somatic cells - Chromosome transfer into zygotes arrested in mitosis Kevin Eggan group: NATURE 2007; 447:2007
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b. Cell fusion reprogramming Science. 2005; 309 : 1369-1373 Dev. Dyn. 2003; 227: 504–510 EMBO J. 1997; 16: 6510–6520 Produced tetraploid hESCs (2005) Comparison of reprogramming efficiency between transduction of reprogramming factors, cell- cell fusion, and cytoplast fusion. Hasegawa K, et al.Stem Cells. 2010 Aug;28(8):1338-48.
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c. Reprogramming by cell extract ???? Hakelien, A. M , et al. Nature Biotechnol. 2002; 20: 460–466. Hansis, C., et al. Curr. Biol. 2004; 14: 1475–1480 Taranger, C. K. et al. Mol. Biol. Cell 2005; 16: 5719–5735. Ratajczak J, et al. Leukemia 2006; 20: 1487–1495
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c. Reprogramming by cell extract Hansis, C., et al. Curr. Biol. 2004; 14: 1475–1480
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c. Reprogramming by cell extract Cho HJ, Lee CS, et al. Blood. 2010 Jul 22;116(3):386-95. Nature Reviews Molecular Cell Biology 2010 Aug; 11: 540-541 Single transfer of mouse ESC-derived proteins (ESC-extract) into primarily cultured adult mouse fibroblasts can achieve full reprogramming to the pluripotent state without the forced expression of ectopic transgenes.
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d. Culture-induced reprogramming Hochedlinger K& Jaenisch R, Nature 2006; 441: 1061-1067
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d. Culture-induced reprogramming Raff, M. Annu. Rev. Cell Dev. Biol. 19, 1–22 (2003). Stuart H. Orkin & Martin Pera, Cell Stem Cell 2007; 1: 271 Adult spermatogonial progenitor cells can give rise to multipotent (pluripotent) adult spermatogonial-derived stem cells (MASCs) . (Marco Seande et al. Nature 2007; 449: 346. Stem Cells. 2009 Jan;27(1):138-149)
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This note was uploaded on 12/18/2011 for the course BCH 4122 taught by Professor Ilonaskerjanc during the Spring '11 term at University of Ottawa.

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Lecture7 - Lecture 7. Epigenetic reprogramming Lisheng WANG...

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