Lecture9 - Lecture 9. Differentiation of human pluripotent...

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Lisheng WANG Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine Lecture 9. Differentiation of human pluripotent stem cells into a given cell type for potential application current progress & Review previous Quiz
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The challenges for clinical application of hESC and human iPSC derivatives Expansion of hESCs Appropriate differentiation Functional integration Risk of teratoma or tumor formation Immune rejection
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Cell replacement therapy Drugs, growth factors, gene transfer, … Stem cells (derived from hESCs and iPSCs) replacement therapy Organ transplantation
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Paul J. Fairchild Immunological aspects in potential hESC therapy Patient-tailored pluriptent stem cells (iPSC, SCNT,…) Differentiation Modified from MSC
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Neuron can be efficiently generated from hESCs and iPSCs (Proc Natl Acad Sci U S A. 2010 Sep 7;107(36):15921-15926. Swistowski A, et al. Stem Cells. 2010 Aug 16 [Epub ahead of print]. Weick JP, et al. Stem Cells. 2010 Sep 8. [Epub ahead of print]). MicroRNA-mediated conversion of human fibroblasts to neurons (Yoo et al, Nature 2011; doi:10.1038/nature10323) Direct generation of functional dopaminergic neurons from human fibroblasts (Caiazzo et al, Nature 2011; doi:10.1038/nature10284) Isolation and directed differentiation of functional neuron from hESCs and iPSCs
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Differentiation of motor neurons (~50%) by a simple sequential application of retinoid acid (RA) and sonic hedgehog (SHH) in a chemically defined suspension culture. Ann N Y Acad Sci. 2010 Jun;1198:192-200 (Review) Li XJ, Hu B, Jones SA. . et al, Stem Cells 2008; 26(4):886-93 Differentiation of Ventral Spinal Progenitors and Motor Neurons from hESC by Small Molecules http://www.etsu.edu/cpah/hsci/forsman/Histology%2 0of%20musclefor%20web_files/image015.jpg
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Toward the generation of rod and cone photoreceptors from mouse, monkey and human ES cells (NATURE BIOTECHNOLOGY 2008; 26: 215-224) Photoreceptors rod corn Pigment epithelium Image from: http://www.usc.edu/dept/gero/AgeWorks/core_courses/gero500_core/biology_b_lect/index_a.htm http://thebrain.mcgill.ca/flash/i/i_02/i_02_cl/i_02_cl_vis/i_02_cl_vis.html
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This note was uploaded on 12/18/2011 for the course BCH 4122 taught by Professor Ilonaskerjanc during the Spring '11 term at University of Ottawa.

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Lecture9 - Lecture 9. Differentiation of human pluripotent...

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