final DGD - BCH 3356 English Thursday 8:30-10:00 TBT333...

Info icon This preview shows pages 1–11. Sign up to view the full content.

View Full Document Right Arrow Icon
BCH 3356 English – Thursday 8:30-10:00 TBT333 English – Friday 2:30-4:00 MNT203 Megan Rose – [email protected]
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Lab book assessment The final lab book assessment will be done at the end of lab 8. Guidelines: Write your full name, the course code, your section and group number, as well as your email, on the front and/or first page of your notebook. If your notebook is not already numbered, number all pages (one side is enough). Devote pages 2 to 4 to a Table of Contents.
Image of page 2
Lab book assessment During the lab session Annotate any required calculations, any variation from the manual protocol, all your readings from instruments (like a spectrophotometer). Record equipment details such as brand and model. Record all your observations Record your measurements, in a table format when appropriate. Have your note book initialed before you
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Final lab report Length Abstract A maximum of half a page Introduction Maximum of 2 pages Materials and methods Maximum of 3 pages Results Maximum of 3 pages excluding the figures. You should present only the important figures.
Image of page 4
Final lab report The final lab report is on the last part of the semester, everything pertaining to the purification of your fusion T7 RNA polymerase Should be prepared according to the guidelines that are provided in A Guide to Writing in the Sciences . Additional suggestions on how to organize and write your report are
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Final exam Final exam counts for 25% of your final mark. The final exam will assess your general understanding of the concepts described in the lab manual and your ability to analyze and interpret experimental results. The content of the final exam will be
Image of page 6
Introduction lab PCR amplification Estimate the concentration of a DNA solution based on its absorbance at 260nm Assess the size and amount of DNA fragments visible on an agarose gel picture Estimate PCR amplification yield by comparing the amount of DNA amplified to the amount of DNA template initially added to the reaction mixture
Image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Analyzing agarose gel results Use a marker with known length (bp) and quantity (ng). Your DNA will migrate in accordance to its length. Use the bands in the marker and compare them to your results in order to estimate the quantity of DNA you have obtained by comparing the INTENSITY of the bands. IMPORTANT: these numbers are based on a loading of 5ul of ladder. If you load 10ul the amounts double!! ng bp
Image of page 8
Lab 1 PCR amplification of fragment of interest (T7 RNA polymerase gene) from parent plasmid. T7 RNA polymerase insert PCR amplification Forward primer Reverse primer
Image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Lab 1 QIAquick spin column Design PCR primers with proper 5’ and 3’ ends for subcloning at specific restriction site(s) within a destination vector Estimate the size and amount of DNA
Image of page 10
Image of page 11
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern