final DGD - BCH 3356 English Thursday 8:30-10:00 TBT333...

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BCH 3356 English – Thursday 8:30-10:00 TBT333 English – Friday 2:30-4:00 MNT203 Megan Rose –
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Lab book assessment The final lab book assessment will be done at the end of lab 8. Guidelines: Write your full name, the course code, your section and group number, as well as your email, on the front and/or first page of your notebook. If your notebook is not already numbered, number all pages (one side is enough).
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Lab book assessment During the lab session Annotate any required calculations, any variation from the manual protocol, all your readings from instruments (like a spectrophotometer). Record equipment details such as brand and model. Record all your observations Record your measurements, in a table format when appropriate.
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Final lab report Length Abstract A maximum of half a page Introduction Maximum of 2 pages Materials and methods Maximum of 3 pages Results Maximum of 3 pages excluding the figures.
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Final lab report The final lab report is on the last part of the semester, everything pertaining to the purification of your fusion T7 RNA polymerase Should be prepared according to the guidelines that are provided in A Guide to Writing in the Sciences . Additional suggestions on how to
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Final exam Final exam counts for 25% of your final mark. The final exam will assess your general understanding of the concepts described in the lab manual and your ability to analyze and interpret experimental results.
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Introduction lab PCR amplification Estimate the concentration of a DNA solution based on its absorbance at 260nm Assess the size and amount of DNA fragments visible on an agarose gel picture Estimate PCR amplification yield by comparing the amount of DNA amplified to the amount of DNA template initially
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Analyzing agarose gel results Use a marker with known length (bp) and quantity (ng). Your DNA will migrate in accordance to its length. Use the bands in the marker and compare them to your results in order to estimate the quantity of DNA you have obtained by comparing the INTENSITY of the bands. IMPORTANT: these numbers are based on a loading of 5ul of ladder. If you load 10ul the amounts double!! ng bp
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Lab 1 PCR amplification of fragment of interest (T7 RNA polymerase gene) from parent plasmid. T7 RNA polymerase insert PCR amplification Forward primer Reverse primer
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Lab 1 QIAquick spin column Design PCR primers with proper 5’ and 3’ ends for subcloning at specific restriction site(s) within a destination vector Estimate the size and amount of DNA fragments on an agarose gel by relative comparison to quantitative DNA markers Refer to the picture of an agarose gel to discuss the specificity of PCR
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This note was uploaded on 12/18/2011 for the course BCH 3356 taught by Professor Odette during the Spring '08 term at University of Ottawa.

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final DGD - BCH 3356 English Thursday 8:30-10:00 TBT333...

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