11 - 02-14-11, Mon. 10:00-11:30 10:00-11:30 am zemin yao...

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Unformatted text preview: 02-14-11, Mon. 10:00-11:30 10:00-11:30 am zemin yao professor and chair biochemistry, microbiology & immunology e-mail: zyao@uottawa.ca BCH 3120 lipid and amino acid metabolism acyl-CoA ACS fatty acid HSCoA + ATP AMP + 2Pi CPT1 carnitine acyl-carnitine HSCoA CPT2 carnitine HSCoA acyl-CoA acyl-carnitine mitochondiral matrix cytosol review lecture 10 1. transport of fatty acids into mitochondria review lecture 10 2. the -oxidation pathway of fatty acyl-CoA acetyl-CoA fatty acyl-CoA (2 C atoms shorter) fatty acyl-CoA trans- 2-enoyl-CoA 3-hydroxyacyl-CoA FAD FADH 2 H 2 O NAD + NADH + H + -ketoacyl-CoA HSCoA AD EH HAD KT 02-09-11, Wed fatty acid oxidation 02-14-11, Mon ketogenesis and fatty acid synthesis 02-16-11, Wed triacylglycerol and phospholipid synthesis uOttawa reading break 02-28-11, Mon biosynthesis of cholesterol 03-02-11, Wed lipoprotein metabolism 03-07-11, Mon regulation of cholesterol homeostasis 03-09-11, Wed amino acid deamination and the urea cycle 03-14-11, Mon biosynthesis of amino acids and related molecules 03-16-11, Wed regulation of amino acid metabolism 03-21-11, Mon midterm course outline FA FA Ileum Jejunum duodenum dietary fat transport into muscle, adipose tissue, and liver via blood stream dietary fat release of FA into muscle & fat tissue blood stream FA FA b l o o d s t r e a m FA release of FA into blood stream during starvation Ketogenesis fatty acid synthesis todays lecture acetyl-CoA TCA cycle mitochondrion acetoacetate -hydroxybutyrate acetone ketogenesis ketone bodies acetoacetate synthesis overview acetyl-CoA acetyl-CoA acetoacetyl-CoA HSCoA HSCoA acetyl-CoA HMG-CoA acetoacetate acetyl-CoA 1 2 3 CH 3- C - SCoA O CH 3- C - SCoA O step 1: 1 st condensation - reversal of the last step in oxidation CH 2- C - SCoA CH 3- C - O O thiolase (acetyl-CoA acetyltransferase) HSCoA acetoacetyl-CoA CH 3- C - SCoA + H 2 O O step 2: 2 nd condensation O- OOC - CH 2 - C - CH 2 - C - SCoA CH 3 OH CH 2- C - SCoA CH 3- C - O O HSCoA HMG-CoA synthase h ydroxy m ethyl g lutaryl-CoA step 3: release of acetoacetate O- OOC - CH 2 - C - CH 2 - C - SCoA CH 3 OH CH 3- C - SCoA O- OOC - CH 2 - C - CH 3 O HMG-CoA lyase acetoacetate reduction of acetoacetate to form -hydroxybutyrate -hydroxybutyrate (C 4 ) -hydroxybutyrate dehydrogenase NADH + H + NAD + acetone (C 3 ) CH 3 - C - CH 3 O CO 2 spontaneous decarboxylation- OOC - CH 2 - C - CH 3 O acetoacetate (C 4 ) - OOC - CH 2 - C - CH 3 OH what do ketone bodies do? ketone bodies are produced by the liver when carbohydrate supply is low ( e.g. fat-rich diet or starvation) under these conditions, acetoacetate and hydroxybutyrate become major energy sources (especially for brain) hyperketonemia , also known as ketoacidosis , is common in diabetes Ketogenesis fatty acid synthesis todays lecture fatty acid (FA) synthesis FA synthesis is the reversal of oxidation precursor, C 2 donor, and ACC fatty acid synthase (FAS) and ACP regulation of FA synthesis...
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11 - 02-14-11, Mon. 10:00-11:30 10:00-11:30 am zemin yao...

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