12 - 02-16-11, Wed. 8:30-10:00 8:30-10:00 am zemin yao...

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Unformatted text preview: 02-16-11, Wed. 8:30-10:00 8:30-10:00 am zemin yao professor and chair biochemistry, microbiology & immunology e-mail: zyao@uottawa.ca BCH 3120 lipid and amino acid metabolism outer membrane inner membrane acetyl-CoA citrate oxaloacetate review lecture 11 1. acetyl-CoA to malonyl-CoA citrate acetyl-CoA oxaloacetate transporter malonyl-CoA ACC fatty acid synthesis occurs in cytosol citrate shuttles acetyl-CoA out of mitochondria, via the tricarboxylate transporter acetyl-CoA is converted to malonyl-CoA by ACC acetyl-CoA acetyl-ACP malonyl-CoA malonyl-ACP HS-CoA HS-ACP acetoacetyl-ACP butyryl-ACP 6 X palmitoyl-ACP 1a 2 1b 4 3 5 H 2 O HS-ACP palmitic acid 6 review lecture 11 2. FAS a megasynthase MAT KS KR DH ER TE NADPH NADP + ACP anchor course outline 02-09-11, Wed fatty acid oxidation 02-14-11, Mon ketogenesis and fatty acid synthesis 02-16-11, Wed triacylglycerol and phospholipid synthesis uOttawa reading break 02-28-11, Mon biosynthesis of cholesterol 03-02-11, Wed lipoprotein metabolism 03-07-11, Mon regulation of cholesterol homeostasis 03-09-11, Wed amino acid deamination and the urea cycle 03-14-11, Mon biosynthesis of amino acids and related molecules 03-16-11, Wed regulation of amino acid metabolism 03-21-11, Mon midterm triacylglycerol synthesis phospholipid synthesis todays lecture general structure of triacylglycerol CH 2- OH HO- CH 2 CH 2- OH glycerol CH 2- O- C- R 1 O R 2- C- O- CH 2 O CH 2- O- C- R 3 O triacylglycerol (TAG) Obesity ancient and new the reactions of TAG biosynthesis in the liver and intestine glucose G3P lysoPA PA DAG DGAT PAP1 TAG AGPAT GPAT MAG CH 2- O- C- R 1 O CH 2- O- C- R 2 O CH 2- OH DAG the key intermediate for TAG synthesis is diacylglycerol (DAG) TAG phospholipids acyl-CoA HSCoA DGAT formation of diacylglycerol (DAG) in the liver CH 2- O- C- R 1 O CH 2- OH DAG CH 2- O- C- R 1 O R 2- C- O- CH 2 O CH 2- O- P- O- phosphatidic acid O O P i PAP1 R 2- C- O- CH 2 O PAP1: phosphatidic acid phosphohydrolase formation of DAG in the intestine is via MAG acylation CH 2- O- C- R 1 O CH 2- OH CH 2- OH CH 2- OH acyl-CoA HSCoA DAG monoacylglycerol (MAG) R 2- C- O- CH 2 O R 2- C- O- CH 2 O PAP2 PAP1 two PAPs in the liver PAP1 translocates from cytosol to endoplasmic reticulum (ER) membrane and becomes activated PAP1 is encoded by the lipin1 gene PAP2 is a membrane protein located on the cell surface ( i.e. plasma membrane) N active inactive more about PAP PAP1 is involved in production of DAG that is ultimately utilized for TAG synthesis PAP2 generates DAG for signal transduction (on the plasma membrane) the PAP1 activity is regulated by translocation between cytosol (inactive) and ER membrane (active) phosphatidic acid CH 2- O- C- R 1 O R 2- C- O- CH 2 CH 2- O- P- O- O O O CH 2- O- C- R 1 O CH 2- O- P- O- O O AGPAT R 2- C- SCoA O HSCoA 1-acylglycerol 3-phosphate (lysophosphatidic acid) GPAT R 1- C- SCoA O HSCoA CH 2- OH HO- CH 2 CH 2- O- P- O- O O glycerol 3-phosphate...
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12 - 02-16-11, Wed. 8:30-10:00 8:30-10:00 am zemin yao...

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